Tumor Necrosis Factor (TNF) (AA 77-233) protein (His tag)

Details zu Produkt Nr. ABIN1047828, Anbieter: Anmelden zum Anzeigen
Proteinname
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Proteineigenschaft
AA 77-233
29
20
12
11
7
5
5
5
4
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Spezies
Human
122
51
30
15
11
8
7
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Quelle
Escherichia coli (E. coli)
166
29
16
4
4
3
3
2
2
2
2
1
1
Protein-Typ
Recombinant
Aufreinigungstag / Konjugat
Dieses Tumor Necrosis Factor Protein ist gelabelt mit His tag.
Applikation
ELISA
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Request

Dieses Produkt umsonst

Schicken Sie uns Ihren Validierungsvorschlag. Ich möchte dieses Produkt validieren

Mehr erfahren

Bilder

Sequenz VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL
Produktmerkmale Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.
Reinheit 90 %
Hintergrund Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
Molekulargewicht 21.4 kD
UniProt P01375
Pathways NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus
Kommentare

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Konzentration 0.2-2 mg/mL
Buffer Tris-based buffer, 50 % glycerol
Handhabung Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week
Lagerung -20 °C
Informationen zur Lagerung Store at -20 °C for extended storage, conserve at -20 °C or -80 °C
Bilder des Herstellers
SDS-PAGE (SDS) image for Tumor Necrosis Factor (TNF) (AA 77-233) protein (His tag) (ABIN1047828) Tumor Necrosis Factor (TNF) (AA 77-233) protein (His tag)
Allgemeine Veröffentlichungen Nedospasov, Shakhov, Turetskaya, Mett, Azizov, Georgiev, Korobko, Dobrynin, Filippov, Bystrov: "Tandem arrangement of genes coding for tumor necrosis factor (TNF-alpha) and lymphotoxin (TNF-beta) in the human genome." in: Cold Spring Harbor symposia on quantitative biology, Vol. 51 Pt 1, pp. 611-24, 1987 (PubMed).

Pennica, Nedwin, Hayflick, Seeburg, Derynck, Palladino, Kohr, Aggarwal, Goeddel: "Human tumour necrosis factor: precursor structure, expression and homology to lymphotoxin." in: Nature, Vol. 312, Issue 5996, pp. 724-9, 1985 (PubMed).

Wang, Creasey, Ladner, Lin, Strickler, Van Arsdell, Yamamoto, Mark: "Molecular cloning of the complementary DNA for human tumor necrosis factor." in: Science (New York, N.Y.), Vol. 228, Issue 4696, pp. 149-54, 1985 (PubMed).

Nedwin, Naylor, Sakaguchi, Smith, Jarrett-Nedwin, Pennica, Goeddel, Gray: "Human lymphotoxin and tumor necrosis factor genes: structure, homology and chromosomal localization." in: Nucleic acids research, Vol. 13, Issue 17, pp. 6361-73, 1985 (PubMed).