NGFB Protein (His tag)

Produktnummer ABIN7520353

Produktdetails

Target: NGFB (Nerve Growth Factor beta (NGFB))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses NGFB Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human Beta-NGF/NGF/NGFB Protein

Sequenz: EPHSESNVPA GHTIPQAHWT KLQHSLDTAL RRARSAPAAA IAARVAGQTR NITVDPRLFK KRRLRSPRVL FSTQPPREAA DTQDLDFEVG GAAPFNRTHR SKRSSSHPIF HRGEFSVCDS VSVWVGDKTT ATDIKGKEVM VLGEVNINNS VFKQYFFETK CRDPNPVDSG CRGIDSKHWN SYCTTTHTFV KALTMDGKQA AWRFIRIDTA CVCVLSRKAV RRA

Spezifität: Glu19-Ala241

Reinheit: > 92 % by SDS-PAGE.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: <0.01EU/μg

Biological Activity Comment: Recombinant Human NGF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 2.2-8.6 ng/mL, corresponding to a specific activity of 1.16x10⁵-4.55x10⁵ units/mg.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Konzentration: 0.43 mg/mL

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Antigendetails

Target: NGFB (Nerve Growth Factor beta (NGFB))

Andere Bezeichnung: Beta-NGF/NGF/NGFB (NGFB Produkte)

Synonyme: NGFB Protein, ngfb Protein, ns:zft386 Protein, xx:zft386 Protein, zgc:109730 Protein, hsan5 Protein, beta-ngf Protein, Ngfb Protein, Beta-NGF Protein, HSAN5 Protein, beta-NGF Protein, nerve growth factor Protein, nerve growth factor b (beta polypeptide) Protein, beta-Nerve growth factor Protein, nerve growth factor (beta polypeptide) Protein, NGF Protein, ngfb Protein, FPV072 Protein, FPV076 Protein, ngf Protein, Ngf Protein

Hintergrund:

Description: NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor?dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Name: NGFB, HSAN5, Beta-NGF

Gen-ID: 4803

UniProt: P01138

Pathways: NF-kappaB Signalweg, RTK Signalweg, Regulation of Cell Size