CRISPR-Cas9 protein (NLS)
Spezies: Streptococcus pyogenes
Wirt: Escherichia coli (E. coli)
Recombinant
GEEN
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- Target
- CRISPR-Cas9
- Protein-Typ
- Recombinant
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Spezies
- Streptococcus pyogenes
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Quelle
- Escherichia coli (E. coli)
- Aufreinigungstag / Konjugat
- NLS
- Applikation
- Genome Editing with Engineered Nucleases (GEEN)
- Verwendungszweck
- Ultra NLS-Cas9-basic
- Produktmerkmale
- Recombinant Streptococcus pyogenes Cas9 gene expressed in an Escherichia coli with a N-terminal nucleic localization signal (NLS).
- Aufreinigung
- Escherichia coli expression system
- Endotoxin-Niveau
- 10 EU/mg as analyzed by gel clotting method
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-
- Applikationshinweise
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CRISPR Genomic editing
The optimal working dilution should be determined by the end user. - Testdurchführung
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Cleavage assay: A 20 uL reaction in 1xCas9 Nuclease Reaction Buffer containing 160 ng linearized plasmid, 100 ng gRNA, and 50 ng Ultra NLS-Cas9-basic for 2 hours at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- In 25 mM Tris, 300 mM NaCl, 0.1 mM EDTA, pH 8.0 (50 % glycerol).
- Konservierungsmittel
- Other preservative
- Lagerung
- -20 °C
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- Target
- CRISPR-Cas9
- Andere Bezeichnung
- Cas9 nuclease
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