CRISPR-Cas9 protein (NLS)

Produktnummer ABIN7509364

Produktdetails

Target: CRISPR-Cas9

Protein-Typ: Recombinant

Spezies: Streptococcus aureus

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: NLS

Applikation: Genome Editing with Engineered Nucleases (GEEN)

Verwendungszweck: SaCas9 2NLS Nuclease

Produktmerkmale: Recombinant Streptococcus aureus tag-free Cas9 nuclease expressed in an Escherichia coli with both N-terminal and C-terminal nucleic localization signal (NLS).

Aufreinigung: Escherichia coli expression system

Endotoxin-Niveau: 10 EU/mg as analyzed by gel clotting method

Anwendungsinformationen

Applikationshinweise: CRISPR Genomic editing
The optimal working dilution should be determined by the end user.

Testdurchführung:

Cleavage assay: A 20 uL reaction in 1xSaCas9 Nuclease Reaction Buffer containing linearized plasmid, gRNA, and SaCas9 2NLS Nuclease for 2 hours at 37°C results in a digestion efficiency of linearized plasmid higher than 90%, as determined by agarose gel electrophoresis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 20 mM Tris, 300 mM NaCl, 0.1 mM TCEP, pH 7.5 (50 % glycerol).

Lagerung: -20 °C

Antigendetails

Target: CRISPR-Cas9

Andere Bezeichnung: Cas9 nuclease