CRISPR-Cas9 (Active) protein (NLS)

Produktnummer ABIN7506276

Produktdetails

Target: CRISPR-Cas9

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Streptococcus pyogenes

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: NLS

Applikation: Genome Editing with Engineered Nucleases (GEEN), Electrophoresis (EP)

Verwendungszweck: Cas9-C-NLS nuclease

Produktmerkmale: Recombinant Streptococcus pyogenes Cas9 (wt) protein expressed in an E. coli with a C-terminal nucleic localization signal (NLS).

Aufreinigung: E. coli expression system

Endotoxin-Niveau: < 0.1 eu/μg

Biological Activity Comment: 20 nM CRISPR/Cas9-C-NLS nuclease incubated for 1 hour at 37℃ result in 90% digestion of the substrate DNA as determined by agarose gel electrophoresis.

Anwendungsinformationen

Applikationshinweise: CRISPR Genome editing
The optimal working dilution should be determined by the end user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50 % Glycerol PH 7.4

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Antigendetails

Target: CRISPR-Cas9

Andere Bezeichnung: Cas9 nuclease