CRISPR-Cas9 (Active) Protein
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- Target
- CRISPR-Cas9
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
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Spezies
- Streptococcus pyogenes
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Quelle
- Escherichia coli (E. coli)
- Applikation
- Genome Editing with Engineered Nucleases (GEEN), Electrophoresis (EP)
- Verwendungszweck
- Cas9 nuclease
- Produktmerkmale
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Recombinant Streptococcus pyogenes Cas9 (wt) protein expressed in an E. coli .
- Aufreinigung
- E. coli expression system
- Biological Activity Comment
- 20 nM CRISPR/Cas9-C-NLS nuclease incubated for 1 hour at 37℃ result in 90% digestion of the substrate DNA as determined by agarose gel electrophoresis.
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- Applikationshinweise
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CRISPR Genome editing
The optimal working dilution should be determined by the end user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Buffer
- In 10 mM Tris, 300 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 50 % Glycerol PH 7.4
- Konservierungsmittel
- Dithiothreitol (DTT)
- Vorsichtsmaßnahmen
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- -20 °C
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- Target
- CRISPR-Cas9
- Andere Bezeichnung
- Cas9 nuclease
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