TNFRSF1B Protein (AA 23-257) (His tag)

Produktnummer ABIN7275760

Produktdetails

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Protein-Typ: Recombinant

Proteineigenschaft: AA 23-257

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses TNFRSF1B Protein ist gelabelt mit His tag.

Verwendungszweck: Human TNFR2/CD120b/TNFRSF1B Protein

Sequenz: Leu23-Asp257

Produktmerkmale: Recombinant Human TNFR2/CD120b/TNFRSF1B Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu23-Asp257.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human TNFR2, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Human TNF alpha, His Tag with the EC50 of 16.6ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B (TNFRSF1B))

Andere Bezeichnung: TNFR2 (TNFRSF1B Produkte)

Synonyme: CD120b Protein, TBPII Protein, TNF-R-II Protein, TNF-R75 Protein, TNFBR Protein, TNFR1B Protein, TNFR2 Protein, TNFR80 Protein, p75 Protein, p75TNFR Protein, TNF-RII/TNFRSF1B Protein, TNFR-II Protein, TNFRSF1B Protein, TNF-R2 Protein, TNF-alphaR2 Protein, TNFRII Protein, TNFalpha-R2 Protein, Tnfr-1 Protein, Tnfr2 Protein, rank Protein, zgc:163064 Protein, TNF receptor superfamily member 1B Protein, tumor necrosis factor receptor superfamily, member 1b Protein, tumor necrosis factor receptor superfamily, member 1B Protein, TNFRSF1B Protein, Tnfrsf1b Protein, tnfrsf1b Protein

Hintergrund: Tumor Necrosis Factor Receptor II (TNF RII), also known as TNFRSF1B, p75/p80, and CD120b, is a type I transmembrane protein that belongs to the TNF receptor superfamily. It has a molecular weight of approximately 75 kDa.Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha. Isoform 2 blocks TNF-alpha-induced apoptosis, which suggests that it regulates TNF-alpha function by antagonizing its biological activity.

Molekulargewicht: 26.2 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.

Pathways: NF-kappaB Signalweg, Apoptose, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Ubiquitin Proteasome Pathway