TIM3 Protein (AA 22-200) (His-Avi Tag,Biotin)

Produktnummer ABIN7275739

Produktdetails

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Protein-Typ: Recombinant

Proteineigenschaft: AA 22-200

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses TIM3 Protein ist gelabelt mit His-Avi Tag,Biotin.

Verwendungszweck: Biotinylated Human Tim-3/HAVCR2 Protein

Sequenz: Ser22-Arg200

Produktmerkmale: Recombinant Biotinylated Human Tim-3/HAVCR2 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Ser22-Arg200.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Anti-Tim-3 Antibody, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human Tim-3, His Tag with the EC50 of 7.6ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Andere Bezeichnung: Tim-3 (TIM 3 Produkte)

Synonyme: HAVCR2 Protein, MGC140131 Protein, HAVcr-2 Protein, KIM-3 Protein, TIM3 Protein, TIMD-3 Protein, TIMD3 Protein, Tim-3 Protein, TIM-3 Protein, Tim3 Protein, Timd3 Protein, tim3 Protein, hepatitis A virus cellular receptor 2 Protein, HAVCR2 Protein, Havcr2 Protein

Hintergrund: Hepatitis A virus cellular receptor 2 (HAVCR2), also known as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), is a protein that in humans is encoded by the HAVCR2 gene.TIM3 is an immune checkpoint and together with other inhibitory receptors including programmed cell death protein 1 (PD-1) and lymphocyte activation gene 3 protein (LAG3) mediate the CD8 T-cell exhaustion. TIM3 has also been shown as a CD4 Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice.

Molekulargewicht: 22.8 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result.

Pathways: Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints