TIM3 Protein (AA 22-200) (His tag,AVI tag,Biotin)

Produktnummer ABIN5674608

Produktdetails

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 22-200

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses TIM3 Protein ist gelabelt mit His tag,AVI tag,Biotin.

Marke: PrecisionAvi

Sequenz: AA 22-200

Spezifität: Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

Produktmerkmale: This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 23.1 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Reinheit: >90 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Kommentare:

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Referenzen

Wu, Li, Xia, Tian, Kong, Wang, Gu, Zhang, Tu, Xie, Yang, Lu, Jiang, Ying: "Identification of Human Single-Domain Antibodies against SARS-CoV-2." in: Cell host & microbe, (2020) (PubMed).

Antigendetails

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Andere Bezeichnung: TIM-3 (TIM 3 Produkte)

Synonyme: HAVCR2 Protein, MGC140131 Protein, HAVcr-2 Protein, KIM-3 Protein, TIM3 Protein, TIMD-3 Protein, TIMD3 Protein, Tim-3 Protein, TIM-3 Protein, Tim3 Protein, Timd3 Protein, tim3 Protein, hepatitis A virus cellular receptor 2 Protein, HAVCR2 Protein, Havcr2 Protein

Substanzklasse: Virus

Hintergrund: Hepatitis A virus cellular receptor 2 is also known as HAVCR2, FLJ14428, KIM3, TIM3, TIMD3, is a member of the TIM family of immune regulating molecules with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells and their associated cytokines are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. The 2 types of cells also cross-regulate the functions of the other. HAVCR2 is a Th1-specific cell surface protein that regulates macrophage activation and enhances the severity of experimental autoimmune encephalomyelitis in mice. HAVCR2 regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Dysregulation of the HAVCR2-galectin-9 pathway could underlie chronic autoimmune disease states in human, such as multiple sclerosis.

Molekulargewicht: 23.1 kDa

NCBI Accession: NP_116171

Pathways: Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints