Sclerostin Protein (SOST) (AA 24-213) (His-Avi Tag)

Produktnummer ABIN7275636

Produktdetails

Target: Sclerostin (SOST)

Protein-Typ: Recombinant

Proteineigenschaft: AA 24-213

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses Sclerostin Protein ist gelabelt mit His-Avi Tag.

Verwendungszweck: Human SOST/Sclerostin Protein

Sequenz: Gln24-Tyr213

Produktmerkmale: Recombinant Human SOST/Sclerostin Protein is expressed from HEK293 with His and Avi tag at the N-Terminus.It contains Gln24-Tyr213.

Reinheit: > 95 % as determined by Tris-Bis PAGE

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human SOST, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-SOST Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Sclerostin (SOST)

Andere Bezeichnung: SOST (SOST Produkte)

Synonyme: LOC100313724 Protein, CDD Protein, VBCH Protein, 5430411E23Rik Protein, sclerostin Protein, LOC100313724 Protein, SOST Protein, Sost Protein

Hintergrund: SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. SOST is negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.

Molekulargewicht: 24.2 kDa. Due to glycosylation,the protein migrates to 28-40 kDa based on Tris-Bis PAGE result.