MICA Protein (His-Avi Tag,Biotin)
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- Target Alle MICA Proteine anzeigen
- MICA (MHC Class I Polypeptide-Related Sequence A (MICA))
- Protein-Typ
- Recombinant
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Spezies
- Human
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Quelle
- HEK-293 Cells
- Aufreinigungstag / Konjugat
- Dieses MICA Protein ist gelabelt mit His-Avi Tag,Biotin.
- Sequenz
- Glu24-Gln308
- Reinheit
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- Sterilität
- 0.22 μm filtered
- Endotoxin-Niveau
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human NKG2D, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human MICA, His Tag with the EC50 of 0.32μg/ml determined by ELISA. See testing image for detail.
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- Buffer
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- Lagerung
- 4 °C,-80 °C
- Informationen zur Lagerung
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- MICA (MHC Class I Polypeptide-Related Sequence A (MICA))
- Andere Bezeichnung
- MICA (MICA Produkte)
- Synonyme
- MIC-A Protein, PERB11.1 Protein, MHC class I polypeptide-related sequence A Protein, MICA Protein
- Hintergrund
- FLJ60820, MGC111087, MICA, PERB11.1, DAMA-345G11.2, FLJ36918, FLJ60820, MGC21250,TMICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85 % amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin.
- Molekulargewicht
- 35.8 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Tris-Bis PAGE result.
- UniProt
- Q96QC4
- Pathways
- Activation of Innate immune Response, Transition Metal Ion Homeostasis
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