MICA Protein (AA 24-308) (His-Avi Tag)

Produktnummer ABIN7275255

Produktdetails

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Protein-Typ: Recombinant

Proteineigenschaft: AA 24-308

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses MICA Protein ist gelabelt mit His-Avi Tag.

Verwendungszweck: Human MICA Protein

Sequenz: Glu24-Gln308

Produktmerkmale: Recombinant Human MICA Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Glu24-Gln308.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human MICA, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.20μg/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: MICA (MHC Class I Polypeptide-Related Sequence A (MICA))

Andere Bezeichnung: MICA (MICA Produkte)

Synonyme: MIC-A Protein, PERB11.1 Protein, MHC class I polypeptide-related sequence A Protein, MICA Protein

Hintergrund: MICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85 % amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin..

Molekulargewicht: 35.8 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.

UniProt: Q96QC4

Pathways: Activation of Innate immune Response, Transition Metal Ion Homeostasis