IL4 Receptor Protein (AA 26-232) (His tag)

Produktnummer ABIN7274989

Produktdetails

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Protein-Typ: Recombinant

Proteineigenschaft: AA 26-232

Spezies: Cynomolgus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses IL4 Receptor Protein ist gelabelt mit His tag.

Verwendungszweck: Cynomolgus IL-4 R alpha/CD124 Protein

Sequenz: Met26-Arg232

Produktmerkmale: Recombinant Cynomolgus IL-4 R alpha/CD124 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Met26-Arg232.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human IL-4, No Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Cynomolgus IL-4 R alpha, His Tag with the EC50 of 36.5ng/ml determined by ELISA. The affinity constant of 13.48 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: IL4 Receptor (IL4R) (Interleukin 4 Receptor (IL4R))

Andere Bezeichnung: IL-4 R alpha (IL4R Produkte)

Synonyme: IL4R Protein, il-4ra Protein, zgc:113063 Protein, CD124 Protein, IL-4RA Protein, IL4RA Protein, Il4ra Protein, IL4 Protein, Il4r Protein, interleukin 4 receptor Protein, interleukin 4 receptor, tandem duplicate 1 Protein, interleukin 4 receptor, alpha Protein, IL4R Protein, il4r.1 Protein, Il4r Protein, Il4ra Protein

Hintergrund: Interleukin 4 Receptor alpha (IL-4 Ra), also known as CD124 and BSF receptor, is a widely expressed 140 kDa transmembrane glycoprotein in the class I cytokine receptor family.The effects of IL-4 are mediated after binding to high affinity receptor complexes present on hematopoietic as well as non-hematopoietic cells.

Molekulargewicht: 24.84 kDa. Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.

UniProt: G7Q0S7

Pathways: JAK-STAT Signalweg, Positive Regulation of Immune Effector Process