PARP1 Protein (His tag)

Produktnummer ABIN7197450

Produktdetails

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses PARP1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human PARP-1 Protein (His Tag)(Active)

Sequenz: Met 1-Trp 1014

Produktmerkmale: The amino acids corresponding to the full length of human PARP1 (NP_001609.2) (Met 1-Trp 1014) was fused with a polyhistidine tag at the C-terminus.

Reinheit: > 90 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1.Measured by its binding ability in a functional ELISA. 2. Immobilized human PARP1 at 10 μg/mL (100 μl/well) can bind biotinylated human HSP70, The EC50 of biotinylated human HSP70 is 0.035 μg/mL.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Andere Bezeichnung: PARP-1 (PARP1 Produkte)

Synonyme: ADPRT Protein, ADPRT 1 Protein, ADPRT1 Protein, ARTD1 Protein, PARP Protein, PARP-1 Protein, PPOL Protein, pADPRT-1 Protein, Adprt Protein, Parp-1 Protein, 5830444G22Rik Protein, AI893648 Protein, Adprp Protein, Adprt1 Protein, C80510 Protein, parp-1 Protein, sPARP-1 Protein, BEST:LD21673 Protein, CG17685 Protein, CG17696 Protein, CG17718 Protein, CG40411 Protein, D.PARP Protein, Dm.pARTa Protein, Dmel\\CG40411 Protein, LD21673.3prime Protein, PARP1 Protein, dPARP Protein, parp Protein, adprt1 Protein, padprt-1 Protein, ppol Protein, si:dkey-206f10.3 Protein, wu:fc60f12 Protein, zgc:110092 Protein, poly(ADP-ribose) polymerase 1 Protein, poly (ADP-ribose) polymerase 1 Protein, poly (ADP-ribose) polymerase family, member 1 Protein, Poly-(ADP-ribose) polymerase Protein, poly(ADP-ribose) polymerase 1 L homeolog Protein, PARP1 Protein, Parp1 Protein, Parp Protein, parp1.L Protein, parp1 Protein

Hintergrund:

Background: Poly (ADP-ribose) polymerase 1(PRAP1), also known as NAD(+) ADP-ribosyltransferase 1(ADPRT), is a chromatin-associated enzyme which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The ADP-D-ribosyl group of NAD+ is transferred to an acceptor carboxyl group on a histone or the enzyme itself, and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. The poly(ADP-ribosyl)ation modification is critical for a wide range of processes, including DNA repair, regulation of chromosome structure, transcriptional regulation, mitosis and apoptosis. PARP1 is demonstrateed to mediate the poly(ADP-ribose) ation of APLF (aprataxin PNK-like factor) and CHFR (checkpoint protein with FHA and RING domains), two representative proteins involved in the DNA damage response and checkpoint regulation. Further, It has been suggested that DNA-dependent protein kinase (DNA-PK), another component of DNA repair, suppresses PARP activity, probably through direct binding and/or sequestration of DNA-ends which serve as an important stimulator for both enzymes. PARP1 inhibitors is thus proposed as a targeted cancer therapy for recombination deficient cancers, such as BRCA2 tumors.

Synonym: ADPRT,ADPRT1,ARTD1,pADPRT-1,PARP,PARP-1,PPOL

Molekulargewicht: 114.5 kDa

NCBI Accession: NP_001609

Pathways: Apoptose, Caspase Kaskade in der Apoptose, DNA Reparatur, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location