PARP1 Protein (AA 2-1014) (His tag)

Produktnummer ABIN3094354

Produktdetails

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 2-1014

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses PARP1 Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequenz: AESSDKLYRV EYAKSGRASC KKCSESIPKD SLRMAIMVQS PMFDGKVPHW YHFSCFWKVG HSIRHPDVEV DGFSELRWDD QQKVKKTAEA GGVTGKGQDG IGSKAEKTLG DFAAEYAKSN RSTCKGCMEK IEKGQVRLSK KMVDPEKPQL GMIDRWYHPG CFVKNREELG FRPEYSASQL KGFSLLATED KEALKKQLPG VKSEGKRKGD EVDGVDEVAK KKSKKEKDKD SKLEKALKAQ NDLIWNIKDE LKKVCSTNDL KELLIFNKQQ VPSGESAILD RVADGMVFGA LLPCEECSGQ LVFKSDAYYC TGDVTAWTKC MVKTQTPNRK EWVTPKEFRE ISYLKKLKVK KQDRIFPPET SASVAATPPP STASAPAAVN SSASADKPLS NMKILTLGKL SRNKDEVKAM IEKLGGKLTG TANKASLCIS TKKEVEKMNK KMEEVKEANI RVVSEDFLQD VSASTKSLQE LFLAHILSPW GAEVKAEPVE VVAPRGKSGA ALSKKSKGQV KEEGINKSEK RMKLTLKGGA AVDPDSGLEH SAHVLEKGGK VFSATLGLVD IVKGTNSYYK LQLLEDDKEN RYWIFRSWGR VGTVIGSNKL EQMPSKEDAI EHFMKLYEEK TGNAWHSKNF TKYPKKFYPL EIDYGQDEEA VKKLTVNPGT KSKLPKPVQD LIKMIFDVES MKKAMVEYEI DLQKMPLGKL SKRQIQAAYS ILSEVQQAVS QGSSDSQILD LSNRFYTLIP HDFGMKKPPL LNNADSVQAK VEMLDNLLDI EVAYSLLRGG SDDSSKDPID VNYEKLKTDI KVVDRDSEEA EIIRKYVKNT HATTHNAYDL EVIDIFKIER EGECQRYKPF KQLHNRRLLW HGSRTTNFAG ILSQGLRIAP PEAPVTGYMF GKGIYFADMV SKSANYCHTS QGDPIGLILL GEVALGNMYE LKHASHISKL PKGKHSVKGL GKTTPDPSAN ISLDGVDVPL GTGISSGVND TSLLYNEYIV YDIAQVNLKY LLKLKFNFKT SLW
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PARP1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: PARP1 (Poly (ADP-Ribose) Polymerase 1 (PARP1))

Andere Bezeichnung: PARP1 (PARP1 Produkte)

Synonyme: ADPRT Protein, ADPRT 1 Protein, ADPRT1 Protein, ARTD1 Protein, PARP Protein, PARP-1 Protein, PPOL Protein, pADPRT-1 Protein, Adprt Protein, Parp-1 Protein, 5830444G22Rik Protein, AI893648 Protein, Adprp Protein, Adprt1 Protein, C80510 Protein, parp-1 Protein, sPARP-1 Protein, BEST:LD21673 Protein, CG17685 Protein, CG17696 Protein, CG17718 Protein, CG40411 Protein, D.PARP Protein, Dm.pARTa Protein, Dmel\\CG40411 Protein, LD21673.3prime Protein, PARP1 Protein, dPARP Protein, parp Protein, adprt1 Protein, padprt-1 Protein, ppol Protein, si:dkey-206f10.3 Protein, wu:fc60f12 Protein, zgc:110092 Protein, poly(ADP-ribose) polymerase 1 Protein, poly (ADP-ribose) polymerase 1 Protein, poly (ADP-ribose) polymerase family, member 1 Protein, Poly-(ADP-ribose) polymerase Protein, poly(ADP-ribose) polymerase 1 L homeolog Protein, PARP1 Protein, Parp1 Protein, Parp Protein, parp1.L Protein, parp1 Protein

Hintergrund: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. {ECO:0000269|PubMed:17177976, ECO:0000269|PubMed:18172500, ECO:0000269|PubMed:19344625, ECO:0000269|PubMed:19661379, ECO:0000269|PubMed:23230272}.

Molekulargewicht: 113.9 kDa Including tag.

UniProt: P09874

Pathways: Apoptose, Caspase Kaskade in der Apoptose, DNA Reparatur, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location