PLK1 Protein (His tag)
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- Target Alle PLK1 Produkte
- PLK1 (Polo-Like Kinase 1 (PLK1))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
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Spezies
- Maus
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Quelle
- Baculovirus infected Insect Cells
- Aufreinigungstag / Konjugat
- Dieses PLK1 Protein ist gelabelt mit His tag.
- Verwendungszweck
- Recombinant Mouse PLK1/PLK-1 Protein (His Tag)(Active)
- Sequenz
- Met 1-Ser 603
- Produktmerkmale
- A DNA sequence encoding the mouse PLK1 (Q07832) (Met 1-Ser 603) was fused with a polyhistidine tag at the N-terminus.
- Reinheit
- > 90 % as determined by SDS-PAGE
- Endotoxin-Niveau
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- The specific activity was determined to be 3 nmol/min/mg using casein as substrate.
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- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Frozen, Liquid
- Buffer
- Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- PLK1 (Polo-Like Kinase 1 (PLK1))
- Andere Bezeichnung
- PLK1/PLK-1 (PLK1 Produkte)
- Synonyme
- PLK Protein, STPK13 Protein, plk Protein, PLK-1 Protein, Plx1 Protein, stpk13 Protein, Plk Protein, cb525 Protein, cb636 Protein, ik:tdsubc_2d1 Protein, wu:fb37g11 Protein, wu:fb76g03 Protein, xx:tdsubc_2d1 Protein, polo like kinase 1 Protein, polo like kinase 1 S homeolog Protein, polo-like kinase 1 Protein, polo-like kinase 1 (Drosophila) Protein, Serine/threonine-protein kinase plk-1 Protein, PLK1 Protein, plk1.S Protein, plk1 Protein, Plk1 Protein, plk-1 Protein
- Hintergrund
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Background: Serine / threonine-protein kinase PLK1 / PLK-1, also known as polo-like kinase 1 (PLK-1) or serine / threonine-protein kinase 13 (STPK13), Polo-like kinases (PLKs), is a family of four serine / threonine protein kinases that are critical regulators of cell cycle progression, mitosis, cytokinesis, and the DNA damage response. PLK1 / PLK-1 is ubiquitously expressed. The mRNA and protein expression of PLK1 / PLK-1, -2 and -4 are coordinately regulated during cell cycle progression, but PLK3 levels are independent of the other three family members. PLK1 / PLK-1 is the most well characterized member of this family and strongly promotes the progression of cells through mitosis. During the various stages of mitosis PLK1 / PLK-1 localizes to the centrosomes, kinetochores and central spindle. PLKs are dysregulated in a variety of human cancers. PLK1 / PLK-1 overexpression correlates with cellular proliferation and poor prognosis. Serine / threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC / C inhibitors, and the regulation of mitotic exit and cytokinesis. It is required for recovery after DNA damage checkpoint and entry into mitosis. PLK1 / PLK-1 is required for kinetochore localization of BUB1B, spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. PLK1 / PLK-1 Phosphorylates BORA, and thereby promotes the degradation of BORA. PLK1 / PLK-1 also contributes to the regulation of AURKA function and phosphorylates SGOL1.
Synonym: Plk,STPK13
- Molekulargewicht
- 70.6 kDa
- UniProt
- Q07832
- Pathways
- Zellzyklus, M Phase
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