PLK1 Protein (His tag)

Produktnummer ABIN7317096

Produktdetails

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses PLK1 Protein ist gelabelt mit His tag.

Verwendungszweck: Recombinant Human PLK1/PLK-1 Protein (His Tag)(Active)

Sequenz: Met 1-Ser 603

Produktmerkmale: A DNA sequence encoding the human PLK1 (NP_005021.2) (Met 1-Ser 603) was expressed, with a polyhistidine tag at the N-terminus.

Reinheit: > 90 % as determined by reducing SDS-PAGE.

Endotoxin-Niveau: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 5 nmol/min/mg using casein as substrate.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Frozen, Liquid

Buffer: Supplied as sterile 50 mM Tris, 100 mM NaCl, pH 7.4, 0.5 mM EDTA, 0.5 mM EGTA, 0.5 mM PMSF, 25 % glycerol

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Antigendetails

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Andere Bezeichnung: PLK1/PLK-1 (PLK1 Produkte)

Synonyme: PLK Protein, STPK13 Protein, plk Protein, PLK-1 Protein, Plx1 Protein, stpk13 Protein, Plk Protein, cb525 Protein, cb636 Protein, ik:tdsubc_2d1 Protein, wu:fb37g11 Protein, wu:fb76g03 Protein, xx:tdsubc_2d1 Protein, polo like kinase 1 Protein, polo like kinase 1 S homeolog Protein, polo-like kinase 1 Protein, polo-like kinase 1 (Drosophila) Protein, Serine/threonine-protein kinase plk-1 Protein, PLK1 Protein, plk1.S Protein, plk1 Protein, Plk1 Protein, plk-1 Protein

Hintergrund:

Background: Serine / threonine-protein kinase PLK1 / PLK-1, also known as polo-like kinase 1 (PLK-1) or serine / threonine-protein kinase 13 (STPK13), Polo-like kinases (PLKs), is a family of four serine / threonine protein kinases that are critical regulators of cell cycle progression, mitosis, cytokinesis, and the DNA damage response. PLK1 / PLK-1 is ubiquitously expressed. The mRNA and protein expression of PLK1 / PLK-1, -2 and -4 are coordinately regulated during cell cycle progression, but PLK3 levels are independent of the other three family members. PLK1 / PLK-1 is the most well characterized member of this family and strongly promotes the progression of cells through mitosis. During the various stages of mitosis PLK1 / PLK-1 localizes to the centrosomes, kinetochores and central spindle. PLKs are dysregulated in a variety of human cancers. PLK1 / PLK-1 overexpression correlates with cellular proliferation and poor prognosis. Serine / threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC / C inhibitors, and the regulation of mitotic exit and cytokinesis. It is required for recovery after DNA damage checkpoint and entry into mitosis. PLK1 / PLK-1 is required for kinetochore localization of BUB1B, spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. PLK1 / PLK-1 Phosphorylates BORA, and thereby promotes the degradation of BORA. PLK1 / PLK-1 also contributes to the regulation of AURKA function and phosphorylates SGOL1.

Synonym: PLK,STPK13

Molekulargewicht: 70.5 kDa

NCBI Accession: NP_005021

Pathways: Zellzyklus, M Phase