CDK1 Protein (GST tag,His tag)

Produktnummer ABIN7195307

Produktdetails

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Protein-Typ: Recombinant

Spezies: Maus

Quelle: Baculovirus infected Insect Cells

Aufreinigungstag / Konjugat: Dieses CDK1 Protein ist gelabelt mit GST tag,His tag.

Verwendungszweck: Recombinant Mouse CDC2 Kinase/CDK1 Protein (His & GST Tag)(Active)

Sequenz: Met1-Met297

Produktmerkmale: A DNA sequence encoding the mouse CDK1 (P11440) (Met1-Met297) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Reinheit: > 85 % as determined by SDS-PAGE

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Kinase activity untested

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM Nacl, 10 % glycerol, pH 8.0

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Antigendetails

Target: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Andere Bezeichnung: CDC2 Kinase/CDK1 (CDK1 Produkte)

Synonyme: CDC2 Protein, CDC28A Protein, P34CDC2 Protein, Cdc2 Protein, Cdc2a Protein, p34 Protein, cdc2 Protein, wu:fc30e01 Protein, zgc:92032 Protein, PSTAIR Protein, cdc-2 Protein, cdc2-a Protein, cdc28a Protein, cdc2x1.1 Protein, p34cdc2 Protein, xcdc2 Protein, 5363 Protein, CDCDm Protein, CDK1 Protein, CDK1/CDC2 Protein, CG5363 Protein, Cdk-1 Protein, Cdk1 Protein, Dcdc2 Protein, Dm cdc2 Protein, DmCdc2 Protein, DmCdk1 Protein, Dmcdc2 Protein, Dmel\\CG5363 Protein, cdc Protein, cdc2Dm Protein, cdk1 Protein, dCdk1 Protein, group 4 Protein, l(2)31Eh Protein, CDC2A Protein, CDC2AAT Protein, CDK2 Protein, CDKA1 Protein, CDKA;1 Protein, CYCLIN-DEPENDENT KINASE A;1 Protein, cell division control 2 Protein, cdc2-b Protein, cdc2a Protein, cdc2x1.2 Protein, POL3 Protein, pold Protein, cyclin dependent kinase 1 Protein, cyclin dependent kinase like 1 Protein, Cell division control protein 2 1 Protein, cyclin-dependent kinase 1 Protein, cyclin-dependent kinase 1 S homeolog Protein, Cyclin-dependent kinase 1 Protein, cell division control 2 Protein, cell division control protein2 homolog Protein, cyclin-dependent kinase 1 L homeolog Protein, polymerase (DNA directed), delta 1, catalytic subunit L homeolog Protein, cyclin-dependent protein kinase Cdk1/Cdc2 Protein, CDK1 Protein, CDKL1 Protein, POPTR_0004s14080g Protein, Cdk1 Protein, cdk1 Protein, cdk1.S Protein, CDC2 Protein, cdc2 Protein, cdk-1 Protein, cdk1.L Protein, pold1.L Protein

Hintergrund:

Background: CDC2, also known as CDK1, contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily. CDC2 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with CDC2 and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDC2 also play important regulatory roles in cell cycle control. It is required in higher cells for entry into S-phase and mitosis. CDC2 also is a cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. It has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. CDK1 is required for RNA splicing, possibly by phosphorylating SRSF1/SF2. It is involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors.

Synonym: Cdc2;CDC2>Cdc2a;p34<

Molekulargewicht: 61.9 kDa

UniProt: P11440

Pathways: Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA