BIRC2 Protein (AVI tag)

Produktnummer ABIN7317660

Produktdetails

Target: BIRC2 (Baculoviral IAP Repeat Containing 2 (BIRC2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses BIRC2 Protein ist gelabelt mit AVI tag.

Verwendungszweck: Recombinant Human cIAP1/HIAP2 Protein (AVI Tag)(Active)

Sequenz: Glu 144-Leu 356

Produktmerkmale: A DNA sequence encoding the BIR2 & BIR3 domains (Glu 144-Leu 356) of human cIAP1 (NP_001157.1) was expressed, fused with the AVI tag at the C-terminus, and two additional amino acids (Gly & Pro) at the N-terminus.

Reinheit: > 92 % as determined by reducing SDS-PAGE.

Biological Activity Comment: Measured by its ability to inhibit DEVD-AFC cleavage activity in cell extracts activated by addition of cytochrome c and dATP. The IC50 for this effect is typically 25-750 nM.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 10 mM Tris, 5 % glycerol, 0.5 mM EDTA, 5 mM DTT, pH 7.5

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Antigendetails

Target: BIRC2 (Baculoviral IAP Repeat Containing 2 (BIRC2))

Andere Bezeichnung: cIAP1/HIAP2 (BIRC2 Produkte)

Synonyme: API1 Protein, HIAP2 Protein, Hiap-2 Protein, MIHB Protein, RNF48 Protein, c-IAP1 Protein, cIAP1 Protein, Api2 Protein, rIAP1 Protein, IAP1 Protein, ITA Protein, AW146227 Protein, Api1 Protein, Birc3 Protein, HIAP1 Protein, IAP2 Protein, MIAP1 Protein, MIAP2 Protein, MIHC Protein, cIAP2 Protein, mcIAP1 Protein, ciap-1 Protein, birc3 Protein, api1 Protein, birc2 Protein, c-iap1 Protein, ciap1 Protein, hiap-2 Protein, hiap2 Protein, mihb Protein, rnf48 Protein, baculoviral IAP repeat containing 2 Protein, baculoviral IAP repeat-containing 2 Protein, baculoviral IAP repeat containing 2 L homeolog Protein, BIRC2 Protein, Birc2 Protein, birc2 Protein, birc2.L Protein

Hintergrund:

Background: The cellular inhibitor of apoptosis protein-1 (cIAP1) is a member of the Inhibitor of Apoptosis family proteinsare (IAP) whose members are characterized by a novel domain of about 70 amino acids termed baculoviral IAP repeats (BIRs). The BIR domains of cIAP1 and cIAP2 bind to caspases, the key effector proteases of apoptosis. The IAP protein family which can enhance cell survival are crucial regulators of programmed cell death. Both cIAP1 and cIAP2 are the E3 ubiquitin protein isopeptide ligases for Smac, taking part in promoting cancer survival through functioning as E3 ubiqitin ligases. Removal of cIAP1 by genetic deletion may result in NF-κB signaling activation that induces TNFα production and in killing sensitive tumor cells through enhanced TNF-R1 death-receptor signaling and caspase 8 activation. The substrate-dependent E3 activity of cIAPs is mediated by their RING domains and is dependent on the specific interactions between cIAPs and Smac. cIAP1 and cIAP2 are also reported to be regulators of NF-kB activation upon TNFαtreatment.

Synonym: API1;c-IAP1;cIAP1;Hiap-2;HIAP2;MIHB;RNF48

Molekulargewicht: 26.5 kDa

NCBI Accession: NP_001157

Pathways: Apoptose, Caspase Kaskade in der Apoptose, Activation of Innate immune Response, Toll-Like Receptors Cascades