GAS6 Protein (AA 136-311) (His tag)

Produktnummer ABIN6239765

Produktdetails

Target: GAS6 (Growth Arrest-Specific 6 (GAS6))

Biologische Aktivität: Active

Proteineigenschaft: AA 136-311

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses GAS6 Protein ist gelabelt mit His tag.

Applikation: Activity Assay (AcA), Cell Culture (CC)

Produktmerkmale: Tag location: N-terminal His Tag

Reinheit: > 90 %

Biological Activity Comment: Growth arrest-specific 6, also known as GAS6, is a gamma-carboxyglutamic acid (Gla) domain-containing protein thought to be involved in the stimulation of cell proliferation. It has been reported that both PC-3 and DU 145 human prostate cancer cell lines are stimulated to proliferate by Gas6, however, this proliferative response strictly correlates with the expression of the Axl receptor, being higher in DU 145 cells. To test the proliferative effect of Gas6, DU 145 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of GAS6. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC. Cell proliferation of DU145 cells after incubation with GAS6 for 72h observed by inverted microscope was shown in Figure 1.

Anwendungsinformationen

Applikationshinweise: Isoelectric Point: 5.6

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Konservierungsmittel: Dithiothreitol (DTT), Other preservative, ProClin

Vorsichtsmaßnahmen: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Antigendetails

Target: GAS6 (Growth Arrest-Specific 6 (GAS6))

Andere Bezeichnung: Growth Arrest Specific Protein 6 (GAS6) (GAS6 Produkte)

Synonyme: fj34a12 Protein, zgc:63860 Protein, wu:fj34a12 Protein, MGC68463 Protein, gas6 Protein, MGC83872 Protein, GAS6 Protein, Gas-6 Protein, AXLLG Protein, AXSF Protein, growth arrest specific 6 Protein, growth arrest-specific 6 Protein, growth arrest specific 6 L homeolog Protein, growth arrest specific 6 S homeolog Protein, GAS6 Protein, gas6 Protein, gas6.L Protein, gas6.S Protein, Gas6 Protein

Hintergrund: Alternative Names: AXLLG, AXSF, AXL Stimulatory Factor, AXL receptor tyrosine kinase ligand

Molekulargewicht: 24kDa

UniProt: Q14393

Pathways: RTK Signalweg, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response