EGF Protein (AA 23-1032) (His tag)

Produktnummer ABIN3092261

Produktdetails

Target: EGF (Epidermal Growth Factor (EGF))

Protein-Typ: Recombinant

Proteineigenschaft: AA 23-1032

Spezies: Human

Quelle: Insektenzellen

Aufreinigungstag / Konjugat: Dieses EGF Protein ist gelabelt mit His tag.

Applikation: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequenz: PQHWSCPEGT LAGNGNSTCV GPAPFLIFSH GNSIFRIDTE GTNYEQLVVD AGVSVIMDFH YNEKRIYWVD LERQLLQRVF LNGSRQERVC NIEKNVSGMA INWINEEVIW SNQQEGIITV TDMKGNNSHI LLSALKYPAN VAVDPVERFI FWSSEVAGSL YRADLDGVGV KALLETSEKI TAVSLDVLDK RLFWIQYNRE GSNSLICSCD YDGGSVHISK HPTQHNLFAM SLFGDRIFYS TWKMKTIWIA NKHTGKDMVR INLHSSFVPL GELKVVHPLA QPKAEDDTWE PEQKLCKLRK GNCSSTVCGQ DLQSHLCMCA EGYALSRDRK YCEDVNECAF WNHGCTLGCK NTPGSYYCTC PVGFVLLPDG KRCHQLVSCP RNVSECSHDC VLTSEGPLCF CPEGSVLERD GKTCSGCSSP DNGGCSQLCV PLSPVSWECD CFPGYDLQLD EKSCAASGPQ PFLLFANSQD IRHMHFDGTD YGTLLSQQMG MVYALDHDPV ENKIYFAHTA LKWIERANMD GSQRERLIEE GVDVPEGLAV DWIGRRFYWT DRGKSLIGRS DLNGKRSKII TKENISQPRG IAVHPMAKRL FWTDTGINPR IESSSLQGLG RLVIASSDLI WPSGITIDFL TDKLYWCDAK QSVIEMANLD GSKRRRLTQN DVGHPFAVAV FEDYVWFSDW AMPSVMRVNK RTGKDRVRLQ GSMLKPSSLV VVHPLAKPGA DPCLYQNGGC EHICKKRLGT AWCSCREGFM KASDGKTCLA LDGHQLLAGG EVDLKNQVTP LDILSKTRVS EDNITESQHM LVAEIMVSDQ DDCAPVGCSM YARCISEGED ATCQCLKGFA GDGKLCSDID ECEMGVPVCP PASSKCINTE GGYVCRCSEG YQGDGIHCLD IDECQLGEHS CGENASCTNT EGGYTCMCAG RLSEPGLICP DSTPPPHLRE DDHHYSVRNS DSECPLSHDG YCLHDGVCMY IEALDKYACN CVVGYIGERC QYRDLKWWEL RHAGHGQQQK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Produktmerkmale:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human EGF Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Aufreinigung: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Reinheit: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Protein is endotoxin free.

Güteklasse: Crystallography grade

Anwendungsinformationen

Applikationshinweise: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Kommentare:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: Unlimited (if stored properly)

Antigendetails

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: EGF (EGF Produkte)

Synonyme: HOMG4 Protein, URG Protein, AI790464 Protein, CEGF Protein, epidermal growth factor Protein, pro-epidermal growth factor Protein, EGF Protein, egf Protein, CpipJ_CPIJ020278 Protein, Egf Protein

Hintergrund: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro (PubMed:10964941). {ECO:0000269|PubMed:10964941, ECO:0000269|PubMed:17671655}.

Molekulargewicht: 113.1 kDa Including tag.

UniProt: P01133

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation