Epidermal Growth Factor (EGF) (Active) Protein

Details zu Produkt Nr. ABIN987821
Proteinname
  • HOMG4
  • URG
  • AI790464
  • CEGF
  • epidermal growth factor
  • pro-epidermal growth factor
  • EGF
  • egf
  • CpipJ_CPIJ020278
  • Egf
Spezies
Human
79
43
24
7
4
1
1
Quelle
Escherichia coli (E. coli)
112
8
5
5
4
4
3
2
2
1
1
1
Biologische Aktivität
Active
Optionen
Sequenz NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW EL
Produktmerkmale The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less then 2 ng/ml, corresponding to a specific activity of 5.0×105 IU/ mg.
Reinheit > 95 % as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel
Endotoxin-Niveau Level Less than 0.1ng/myg (1 IEU/myg) determined by LAL test
Proteinname
Hintergrund Human Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Human Epidermal Growth Factor (EGF) is a 6,200 Da protein containing 53 amino acid residues. Synonym: rHuEGF, Epidermal Growth Facto. Formulation: The protein was lyophilized after extensive dialysis against 10mM Phosphate buffer, pH7.0, 200mM NaCl buffer.
Molekulargewicht 6.0 kDa+/-10% determined by reduced SDS-PAGE Isoelectric Point The main zone between 4.0-5.0 analysis by IEF UV Scan The maximal absorption wave is 275+/-3 nm.
Pathways NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution It is recommended to reconstitute the lyophilized Recombinant Human Epidermal Growth Factor (EGF) in sterile 18 Mohm-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. Quantitation Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).2. RP-HPLC analysis, using a calibrated solution of EGF as a reference standard.
Lagerung -20 °C
Bilder des Herstellers
SDS-PAGE (SDS) image for Epidermal Growth Factor (EGF) (Active) protein (ABIN987821) 2 μg of EGF, Human was resolved with SDS-PAGE under reducing (R) and non-reducing (N...
 image for Epidermal Growth Factor (EGF) (Active) protein (ABIN987821) Epidermal Growth Factor (EGF) (Active) protein (Image 2)
Activity Assay (AcA) image for Epidermal Growth Factor (EGF) (Active) protein (ABIN987821) EGF, Human stimulates cell proliferation of the Balb/3T3 Cells. The ED50 for this eff...
Produkt verwendet in: Li, Niu, Lang, Guo, Ma, Kiesewetter, Backer, Shen, Chen: "PET imaging of EGF receptors using [18F]FBEM-EGF in a head and neck squamous cell carcinoma model." in: European journal of nuclear medicine and molecular imaging, Vol. 39, Issue 2, pp. 300-8, 2012 (PubMed).

Allgemeine Veröffentlichungen Katagiri, Takahashi: "Potential relationship between normalization of endometrial epidermal growth factor profile and restoration of fertility in repeat breeder cows." in: Animal reproduction science, Vol. 95, Issue 1-2, pp. 54-66, 2006 (PubMed).

Endo, Sasaki, Yano, Kobayashi, Yukiue, Haneda, Suzuki, Kawano, Fujii: "Evaluation of the epidermal growth factor receptor gene mutation and copy number in non-small cell lung cancer with gefitinib therapy." in: Oncology reports, Vol. 16, Issue 3, pp. 533-41, 2006 (PubMed).