CD40 Protein (CD40) (AA 24-193) (His tag)

Produktnummer ABIN2870723

Produktdetails

Target: CD40

Protein-Typ: Recombinant

Biologische Aktivität: Active

Proteineigenschaft: AA 24-193

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses CD40 Protein ist gelabelt mit His tag.

Sequenz: AA 24-193

Produktmerkmale: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.8 kDa. The protein migrates as 30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Reinheit: >95 % as determined by SDS-PAGE.

Endotoxin-Niveau: Less than 1.0 EU per μg by the LAL method.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4

Handhabung: Please avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Referenzen

Aghevlian, Wu, Raie, Tumbale, Kare, Seo, Ferrara: "Pre-clinical evaluation of immunoPET imaging using agonist CD40 monoclonal antibody in pancreatic tumor-bearing mice." in: Nuclear medicine and biology, Vol. 98-99, pp. 8-17 (PubMed).

Antigendetails

Target: CD40

Andere Bezeichnung: CD40 (CD40 Produkte)

Synonyme: Bp50 Protein, CDW40 Protein, TNFRSF5 Protein, p50 Protein, AI326936 Protein, GP39 Protein, HIGM1 Protein, IGM Protein, IMD3 Protein, T-BAM Protein, TRAP Protein, Tnfrsf5 Protein, TNFSF5 Protein, CD40 molecule Protein, CD40 antigen Protein, CD40 Protein, Cd40 Protein

Hintergrund: CD40 is also known as TNFRSF5, Bp50, CDW40, MGC9013, TNFRSF5 and p50, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins, and plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 contains 4 cysteine-rich repeats in the extracellular domain, and is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. The extracellular domain has the cysteinerich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to aggregation of CD40 Molecules,which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3), an autosomal recessive disorder characterized by an inability of B cells to undergo isotype switching, as well as an inability to mount an antibody-specific immune response, and a lack of germinal center formation.

Molekulargewicht: 20.8 kDa

UniProt: P27512

Pathways: NF-kappaB Signalweg, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints