CNTF Protein (AA 2-200)
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- Target Alle CNTF Proteine anzeigen
- CNTF (Ciliary Neurotrophic Factor (CNTF))
- Protein-Typ
- Recombinant
- Biologische Aktivität
- Active
- Proteineigenschaft
- AA 2-200
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Spezies
- Human
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Quelle
- Escherichia coli (E. coli)
- Applikation
- Biochemical Assay (BCA)
- Reinheit
- >98 % , as determined by Coomassie stained SDS-PAGE.
- Endotoxin-Niveau
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Less than 0.1 ng per μg of protein.
- Top Product
- Discover our top product CNTF Protein
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- Applikationshinweise
- Optimal working dilution should be determined by the investigator.
- Kommentare
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Biological activity: ED50 = 50 - 150 ng/ml, corresponding to a specific activity of 0.67 - 2.0 x 104 units/mg, as measured by its ability to stimulate proliferation of human TF-1 cells in a dose dependent manner.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- For maximum results, quick spin vial prior to opening. Reconstitute in water containing BSA (50 μg of BSA per 1 μg of protein) to a concentration of 0.1-1.0 mg/mL. Do not vortex. It is recommended to further dilute in a buffer containing a carrier protein such as 0.1 % BSA and store working aliquots at -20 °C to -80 °C.
- Buffer
- Lyophilized, carrier-free.
- Handhabung
- Avoid repeated freeze/thaw cycles.
- Lagerung
- -20 °C
- Informationen zur Lagerung
- Unopened vial can be stored at -20°C or -70°C.
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- Target
- CNTF (Ciliary Neurotrophic Factor (CNTF))
- Andere Bezeichnung
- CNTF (CNTF Produkte)
- Synonyme
- GPA Protein, CNTF Protein, HCNTF Protein, AI429687 Protein, ciliary neurotrophic factor Protein, CNTF Protein, Cntf Protein
- Hintergrund
- CNTF is a member of the four-helix bundle cytokine family and is structurally related to other family members such as IL-6, IL-11, and LIF. Binding of CNTF to its receptor CNTFRα triggers the association with the IL-6 receptor gp130 and the LIF receptor LIFRβ, followed by activating the JAK-STAT, the ERK, and the PI3K signaling pathways. CNTF was first identified as a neuroprotective factor that supported the survival of embryonic chick ciliary neurons. CNTF has been shown to regulate the differentiation and survival of a variety of neuronal cells and has been applied as the treatment for retinal degenerative diseases caused by genetic defect or damage induced by toxins, auto-antibodies, or strong light. CNTF is also revealed as a myotrophic factor for denervated skeletal muscle and a modulator for AMPK-mediated energy balance control.
- Molekulargewicht
- The 199 amino acid recombinant protein has a predicted molecular mass of approximately 22.8 kDa. The predicted N-terminal amino acid is Ala.
- Pathways
- JAK-STAT Signalweg
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