CNTF Protein (AA 4-196, partial) (His tag)

Produktnummer ABIN1095544

Produktdetails

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Protein-Typ: Recombinant

Proteineigenschaft: AA 4-196, partial

Spezies: Human

Quelle: Escherichia coli (E. coli)

Aufreinigungstag / Konjugat: Dieses CNTF Protein ist gelabelt mit His tag.

Applikation: ELISA

Sequenz: TEHSPLTPHR RDLCSRSIWL ARKIRSDLTA LTESYVKHQG LNKNINLDSA DGMPVASTDQ WSELTEAERL QENLQAYRTF HVLLARLLED QQVHFTPTEG DFHQAIHTLL LQVAAFAYQI EELMILLEYK IPRNEADGMP INVGDGGLFE KKLWGLKVLQ ELSQWTVRSI HDLRFISSHQ TGIPARGSHY IAN

Produktmerkmale: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Reinheit: 90 %

Anwendungsinformationen

Kommentare:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handhabung: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

Referenzen

Negro, Tolosano, Skaper, Martini, Callegaro, Silengo, Fiorini, Altruda: "Cloning and expression of human ciliary neurotrophic factor." in: European journal of biochemistry / FEBS, Vol. 201, Issue 1, pp. 289-94, (1991) (PubMed).

Masiakowski, Liu, Radziejewski, Lottspeich, Oberthuer, Wong, Lindsay, Furth, Panayotatos: "Recombinant human and rat ciliary neurotrophic factors." in: Journal of neurochemistry, Vol. 57, Issue 3, pp. 1003-12, (1991) (PubMed).

Lam, Fuller, Miller, Kloss, Manthorpe, Varon, Cordell: "Sequence and structural organization of the human gene encoding ciliary neurotrophic factor." in: Gene, Vol. 102, Issue 2, pp. 271-6, (1991) (PubMed).

Antigendetails

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Andere Bezeichnung: Ciliary neurotrophic factor protein (CNTF Produkte)

Synonyme: GPA Protein, CNTF Protein, HCNTF Protein, AI429687 Protein, ciliary neurotrophic factor Protein, CNTF Protein, Cntf Protein

Hintergrund: CNTF is a survival factor for various neuronal cell types. Seems to prevent the degeneration of motor axons after axotomy.

Molekulargewicht: 26.2 kD

UniProt: P26441

Pathways: JAK-STAT Signalweg