anti-Hund Dynactin 1 Antikörper für Immunofluorescence

Recommended Dynactin 1 Antibody (geliefert von: Anmelden zum Anzeigen )

Antigen
Dynactin 1 (DCTN1) Antikörper
  • DAP-150
  • DP-150
  • P135
  • Chip150
  • DCTN1
  • DKFZp469C1813
  • AL022633
  • Glued
  • p150
  • p150
  • CG9206
  • DYNA_DROME
  • Dmel\\CG9206
  • FCP-D
  • Glu
  • P150
  • P150[Glued]
  • p150/Glued
  • p150/glued
  • p150[Glued]
  • p150[glued]
  • t1
  • Dynactin-1
  • glued
  • dynactin subunit 1
  • dynactin 1
  • Dynactin 1, p150 subunit
  • dynactin subunit 1 L homeolog
  • DCTN1
  • Dctn1
  • DCTN1-p150
  • dctn1.L
Epitop
AA 3-202
11
6
5
4
4
3
2
2
1
1
1
1
1
1
1
Reaktivität
Hund, Human, Maus, Ratte (Rattus)
79
48
42
6
5
5
4
3
2
2
2
2
1
1
1
Wirt
Maus
50
18
9
5
Klonalität
Monoklonal
Konjugat
Dieser Dynactin 1 Antikörper ist unkonjugiert
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
BioImaging (BI), Immunofluorescence (IF), Western Blotting (WB)
66
24
21
16
13
11
8
8
2
1
1
1
1
1
1
Optionen
Hersteller
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Bilder

Produktnummer ABIN968918
Preis und Verfügbarkeit auf Anfrage.
Relevance Score ABIN Application Konjugat Host Isotype Epitope Hersteller Clonality References Details
11.745464 ABIN968919 BI IF WB Mouse IgG1 AA 3-202 Anmelden zum Anzeigen 12-P150GLUED 2
1 ABIN967996 IF IHC (zinc) IP WB Mouse IgG1 AA 3-202 Anmelden zum Anzeigen 1-p150Glued 5
1 ABIN967995 IF IHC (zinc) IP WB Mouse IgG1 AA 3-202 Anmelden zum Anzeigen 1-p150Glued 5

General

Antigen Dynactin 1 (DCTN1) Antikörper
Epitop AA 3-202
(11), (6), (5), (4), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reaktivität Hund, Human, Maus, Ratte (Rattus)
(79), (48), (42), (6), (5), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1)
Wirt Maus
(50), (18), (9), (5)
Klonalität (Klon)
Monoklonal   ( )
Konjugat Dieser Dynactin 1 Antikörper ist unkonjugiert
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Applikation BioImaging (BI), Immunofluorescence (IF), Western Blotting (WB)
(66), (24), (21), (16), (13), (11), (8), (8), (2), (1), (1), (1), (1), (1), (1)
Pubmed 2 Publikationen vorhanden
Hersteller Anmelden zum Anzeigen

Produktdetails anti-Dynactin 1 Antikörper

Target Details Dynactin 1 Anwendungsinformationen Handhabung Referenzen für anti-Dynactin 1 Antikörper (ABIN968918) Bilder
Kreuzreaktivität Maus, Human, Hund
Produktmerkmale 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Reinigung The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Rat p150 [Glued] aa. 3-202
Klon 12-P150GLUED
Isotyp IgG1

Target Details Dynactin 1

Produktdetails anti-Dynactin 1 Antikörper Anwendungsinformationen Handhabung Referenzen für anti-Dynactin 1 Antikörper (ABIN968918) Bilder zurück nach oben
Antigen
Andere Bezeichnung p150 Glued (DCTN1 Antibody Abstract)
Hintergrund P150 [Glued] was identified as a polypeptide associated with cytoplasmic dynein,the minus-end-directed microtubule-based motor protein. p150 [Glued] is also a member of the oligomeric dynactin complex. Dynactin mediates dynein-driven vesicle motility, as well as lower eukaryote nuclear transport. p150 [Glued] bears significant homology to the product of the Glued gene in Drosophila. It has been shown in vitro to be a required activator of dynein-mediated transport along microtubules. The p150 [Glued] component of the dynactin complex binds to microtubules and the actin-like protein Centractin (Arp-1), another member of the dynactin complex. In the developing rat, p150 [Glued] is expressed at high levels in neural tissue. Microtubule binding assays with selected constructs of p150 [Glued] indicate that amino acids 39-150 are required for microtubule association.
Molekulargewicht 150 kDa
Pathways M Phase, ER-Nucleus Signaling

Anwendungsinformationen

Produktdetails anti-Dynactin 1 Antikörper Target Details Dynactin 1 Handhabung Referenzen für anti-Dynactin 1 Antikörper (ABIN968918) Bilder zurück nach oben
Applikationshinweise Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.

Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.
Kommentare

Related Products: ABIN968545, ABIN967389

Beschränkungen Nur für Forschungszwecke einsetzbar

Handhabung

Produktdetails anti-Dynactin 1 Antikörper Target Details Dynactin 1 Anwendungsinformationen Referenzen für anti-Dynactin 1 Antikörper (ABIN968918) Bilder zurück nach oben
Format Liquid
Konzentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung -20 °C
Informationen zur Lagerung Store undiluted at -20° C.

Referenzen für anti-Dynactin 1 Antikörper (ABIN968918)

Produktdetails anti-Dynactin 1 Antikörper Target Details Dynactin 1 Anwendungsinformationen Handhabung Bilder zurück nach oben
Produkt verwendet in:

Waterman-Storer, Karki, Holzbaur: "The p150Glued component of the dynactin complex binds to both microtubules and the actin-related protein centractin (Arp-1)." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 5, pp. 1634-8, 1995

Holzbaur, Hammarback, Paschal, Kravit, Pfister, Vallee: "Homology of a 150K cytoplasmic dynein-associated polypeptide with the Drosophila gene Glued." in: Nature, Vol. 351, Issue 6327, pp. 579-83, 1991

Bilder

Produktdetails anti-Dynactin 1 Antikörper Target Details Dynactin 1 Anwendungsinformationen Handhabung Referenzen für anti-Dynactin 1 Antikörper (ABIN968918) zurück nach oben
Bilder des Herstellers
Western Blotting (WB) image for anti-Dynactin 1 (DCTN1) (AA 3-202) antibody (ABIN968918) Western blot analysis of p150 [Glued] on a rat cerebrum lysate (left). Lane 1: 1:1000...
Immunofluorescence (IF) image for anti-Dynactin 1 (DCTN1) (AA 3-202) antibody (ABIN968918) Immunofluorescent staining of SK-N-SH cells (Human neuroblastoma, ATCC HTB-11) (right...