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Dynactin 1 Antikörper (AA 3-202)

DCTN1 Reaktivität: Human, Maus, Ratte, Hund WB, IF, BI Wirt: Maus Monoclonal 12-P150GLUED unconjugated
Produktnummer ABIN968918
  • Target Alle Dynactin 1 (DCTN1) Antikörper anzeigen
    Dynactin 1 (DCTN1)
    Bindungsspezifität
    • 15
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 3-202
    Reaktivität
    • 55
    • 22
    • 16
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte, Hund
    Wirt
    • 39
    • 13
    • 3
    • 1
    Maus
    Klonalität
    • 43
    • 13
    Monoklonal
    Konjugat
    • 35
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Dynactin 1 Antikörper ist unkonjugiert
    Applikation
    • 47
    • 17
    • 13
    • 13
    • 13
    • 8
    • 8
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI)
    Kreuzreaktivität
    Maus, Human, Hund
    Produktmerkmale
    1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
    2. Please refer to us for technical protocols.
    3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
    4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
    Aufreinigung
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    Immunogen
    Rat p150 [Glued] aa. 3-202
    Klon
    12-P150GLUED
    Isotyp
    IgG1
    Top Product
    Discover our top product DCTN1 Primärantikörper
  • Applikationshinweise
    Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.

    Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.
    Kommentare

    Related Products: ABIN968545, ABIN967389

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    250 μg/mL
    Buffer
    Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store undiluted at -20° C.
  • Waterman-Storer, Karki, Holzbaur: "The p150Glued component of the dynactin complex binds to both microtubules and the actin-related protein centractin (Arp-1)." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 5, pp. 1634-8, (1995) (PubMed).

    Holzbaur, Hammarback, Paschal, Kravit, Pfister, Vallee: "Homology of a 150K cytoplasmic dynein-associated polypeptide with the Drosophila gene Glued." in: Nature, Vol. 351, Issue 6327, pp. 579-83, (1991) (PubMed).

  • Target
    Dynactin 1 (DCTN1)
    Andere Bezeichnung
    p150 Glued (DCTN1 Produkte)
    Synonyme
    DAP-150 antikoerper, DP-150 antikoerper, P135 antikoerper, Chip150 antikoerper, DCTN1 antikoerper, DKFZp469C1813 antikoerper, AL022633 antikoerper, Glued antikoerper, p150 antikoerper, p150 antikoerper, CG9206 antikoerper, DYNA_DROME antikoerper, Dmel\\CG9206 antikoerper, FCP-D antikoerper, Glu antikoerper, P150 antikoerper, P150[Glued] antikoerper, p150/Glued antikoerper, p150/glued antikoerper, p150[Glued] antikoerper, p150[glued] antikoerper, t1 antikoerper, Dynactin-1 antikoerper, glued antikoerper, dynactin subunit 1 antikoerper, dynactin 1 antikoerper, Dynactin 1, p150 subunit antikoerper, dynactin subunit 1 L homeolog antikoerper, DCTN1 antikoerper, Dctn1 antikoerper, DCTN1-p150 antikoerper, dctn1.L antikoerper
    Hintergrund
    P150 [Glued] was identified as a polypeptide associated with cytoplasmic dynein,the minus-end-directed microtubule-based motor protein. p150 [Glued] is also a member of the oligomeric dynactin complex. Dynactin mediates dynein-driven vesicle motility, as well as lower eukaryote nuclear transport. p150 [Glued] bears significant homology to the product of the Glued gene in Drosophila. It has been shown in vitro to be a required activator of dynein-mediated transport along microtubules. The p150 [Glued] component of the dynactin complex binds to microtubules and the actin-like protein Centractin (Arp-1), another member of the dynactin complex. In the developing rat, p150 [Glued] is expressed at high levels in neural tissue. Microtubule binding assays with selected constructs of p150 [Glued] indicate that amino acids 39-150 are required for microtubule association.
    Molekulargewicht
    150 kDa
    Pathways
    M Phase, ER-Nucleus Signaling
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