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Src ELISA Kit
Src
Reaktivität: Human
pTyr419
Colorimetric
Sandwich ELISA
Cell Lysate, Tissue Lysate
Produktnummer ABIN6951659
Produktdetails Src ELISA Kit
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Target
Alle Src ELISA Kits anzeigen
Src
(Proto-oncogene tyrosine-protein kinase Src (Src))
Bindungsspezifität
pTyr419
Reaktivität
Alle Reaktivitäten für Src ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Human Phospho-SRC (Y419) Quantitative ELISA Kit. This assay quantitatively measures phosphorylated SRC (Tyr419) in lysate samples.
Proben
Cell Lysate, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
The antibody pair provided in this kit recognizes human SRC phosphorylated at site tyrosine 419.
Produktmerkmale
Rapidly measure phosphorylated protein in lysates Screen numerous different cell lysates without performing a Western Blot analysis Minimal hands-on time, convenient, and non-radioactive material
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Anti-Phospho Antibody HRP-Conjugated Secondary Antibody Assay Diluent TMB One-Step Substrate Stop Solution Lysis Buffer Positive Control Sample
Benötigtes Material
Distilled or deionized water 100 mL and 1 liter graduated cylinders Tubes to prepare sample dilutions Protease and Phosphatase inhibitors Precision pipettes to deliver 2 μL to 1 mL volumes Adjustable 1-25 mL pipettes for reagent preparation Benchtop rocker or shaker Microplate reader capable of measuring absorbance at 450 nm
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Anwendungsinformationen
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Applikationshinweise
Optimal working dilution should be determined by the investigator.
Plattentyp
Pre-coated
Protokoll
Prepare all reagents and samples as instructed in the manual. Add 100 μL of sample or positive control to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared primary antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared 1X HRP-Streptavidin to each well. Incubate 1 h at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
Haltbarkeit
6 months
Details zu Src
(ausblenden)
Target
Alle Src ELISA Kits anzeigen
Src
(Proto-oncogene tyrosine-protein kinase Src (Src))
Andere Bezeichnung
SRC (Src Produkte )
Synonyme
AW259666 ELISA Kit, pp60c-src ELISA Kit, C-src1 ELISA Kit, CG7524 ELISA Kit, D-Src64B ELISA Kit, D-src ELISA Kit, DSRC64 ELISA Kit, DSrc ELISA Kit, DSrc64 ELISA Kit, DSrc64B ELISA Kit, Dm SRC1 ELISA Kit, DmHD-358 ELISA Kit, Dmel\CG7524 ELISA Kit, Dsrc ELISA Kit, Dsrc64 ELISA Kit, Dsrc64B ELISA Kit, HD-358 ELISA Kit, MRE5 ELISA Kit, SRC 64B ELISA Kit, Src ELISA Kit, Src1 ELISA Kit, Src64 ELISA Kit, c-src ELISA Kit, dSrc ELISA Kit, dsrc ELISA Kit, src ELISA Kit, src1 ELISA Kit, src64 ELISA Kit, src64B ELISA Kit, src64b ELISA Kit, ASV ELISA Kit, SRC1 ELISA Kit, c-SRC ELISA Kit, p60-Src ELISA Kit, c-Src ELISA Kit, PP60C-SCR ELISA Kit, SDR ELISA Kit, SRC proto-oncogene, non-receptor tyrosine kinase ELISA Kit, uncharacterized LOC5574802 ELISA Kit, proto-oncogene tyrosine-protein kinase src ELISA Kit, Rous sarcoma oncogene ELISA Kit, Src oncogene at 64B ELISA Kit, SRC ELISA Kit, LOC5574802 ELISA Kit, CpipJ_CPIJ010659 ELISA Kit, Src ELISA Kit, Src64B ELISA Kit
Substanzklasse
Viral Protein
Gen-ID
6714
UniProt
P12931
Pathways
JAK-STAT Signalweg , Neurotrophin Signalübertragung , Intracellular Steroid Hormone Receptor Signaling Pathway , Regulation of Intracellular Steroid Hormone Receptor Signaling , Cellular Response to Molecule of Bacterial Origin , Cell-Cell Junction Organization , Regulation of Carbohydrate Metabolic Process , Autophagie , CXCR4-mediated Signaling Events , Signaling Events mediated by VEGFR1 and VEGFR2 , Smooth Muscle Cell Migration , Negative Regulation of intrinsic apoptotic Signaling , Platelet-derived growth Factor Receptor Signaling , Thromboxane A2 Receptor Signaling , Signaling of Hepatocyte Growth Factor Receptor , VEGF Signaling
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