IL1A ELISA Kit

Produktnummer ABIN5026929

Produktdetails IL1A ELISA Kit

Target: IL1A (Interleukin 1 alpha (IL1A))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 3.9-250 pg/mL

Untere Nachweisgrenze: 3.9 pg/mL

Applikation: ELISA

Verwendungszweck: This immunoassay kit allows for the in vitro quantitative determination of Human IL-1Alpha concentrations in serum, plasma and other biological fluids.

Proben: Plasma, Serum

Analytische Methode: Quantitative

Sensitivität: < 2.34 pg/mL

Bestandteile: plate, standard, diluent, antibodies

Benötigtes Material: pipettes, tubes, reader, incubator

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Protokoll: This This assay employs an antibody specific for anti-human IL-1Alpha coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1Alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-humanIL-1Alpha antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1Alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: protect your eyes

Lagerung: 4 °C

Details zu IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Andere Bezeichnung: IL-1alpha (Interleukin 1 Alpha) (IL1A Produkte)

Synonyme: IL-1A ELISA Kit, IL1 ELISA Kit, IL1-ALPHA ELISA Kit, IL1F1 ELISA Kit, IL-6 ELISA Kit, Il-1a ELISA Kit, IL-1 alpha ELISA Kit, IL-1alpha ELISA Kit, L1A ELISA Kit, interleukin 1 alpha ELISA Kit, interleukin 6 ELISA Kit, IL1A ELISA Kit, IL6 ELISA Kit, Il1a ELISA Kit

Pathways: NF-kappaB Signalweg, Autophagie, Cancer Immune Checkpoints