IGF1 ELISA Kit

Produktnummer ABIN4885749

Produktdetails IGF1 ELISA Kit

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Mouse IGF-1 SpeedELISA Kit.

Marke: SpeedELISA

Proben: Plasma, Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Produktmerkmale:

• Compressed workflow with only one wash step and three incubation steps
• 3 hours total processing time
• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Streptavidin-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Capture Antibody
• HRP Conjugated Detection Antibody
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 70 μL of the Antibody Cocktail to each well.
3. Incubate 10 minutes at RT.
4. Add 50 μL of sample or standard to each well.
5. Incubate 2 h at RT.
6. Wash Wells
7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
8. Incubate 30 min at RT.
9. Add 50 μL of Stop Solution to each well.
10. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 6 months

Details zu IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Andere Bezeichnung: IGF-1 (IGF1 Produkte)

Synonyme: IGF-I ELISA Kit, IGF1A ELISA Kit, IGFI ELISA Kit, C730016P09Rik ELISA Kit, Igf-1 ELISA Kit, Igf-I ELISA Kit, Npt2B ELISA Kit, IGF-1 ELISA Kit, IGFIA ELISA Kit, IGF-IB ELISA Kit, igf1 ELISA Kit, IGF-1L ELISA Kit, IGF-1a ELISA Kit, IGF1 ELISA Kit, igf-1 ELISA Kit, igf1-A ELISA Kit, igf1.S ELISA Kit, xigf1 ELISA Kit, insulin like growth factor 1 ELISA Kit, insulin-like growth factor 1 ELISA Kit, insulin-like growth factor I ELISA Kit, insulin like growth factor 1 L homeolog ELISA Kit, IGF1 ELISA Kit, Igf1 ELISA Kit, LOC100136741 ELISA Kit, igf1 ELISA Kit, LOC678666 ELISA Kit, igf1.L ELISA Kit, LOC104911603 ELISA Kit

Gen-ID: 16000

UniProt: P05017

Pathways: RTK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation