CD130/gp130 ELISA Kit

Produktnummer ABIN4885339

Produktdetails CD130/gp130 ELISA Kit

Target: CD130/gp130 (IL6ST) (Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human gp130 (sgp130) SpeedELISA Kit.

Marke: SpeedELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Compressed workflow with only one wash step and three incubation steps
• 3 hours total processing time
• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Streptavidin-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Capture Antibody
• HRP Conjugated Detection Antibody
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 70 μL of the Antibody Cocktail to each well.
3. Incubate 10 minutes at RT.
4. Add 50 μL of sample or standard to each well.
5. Incubate 2 h at RT.
6. Wash Wells
7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
8. Incubate 30 min at RT.
9. Add 50 μL of Stop Solution to each well.
10. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 6 months

Details zu CD130/gp130

Target: CD130/gp130 (IL6ST) (Interleukin 6 Signal Transducer (Gp130, Oncostatin M Receptor) (IL6ST))

Andere Bezeichnung: Gp130 (IL6ST Produkte)

Synonyme: CD130 ELISA Kit, CDW130 ELISA Kit, GP130 ELISA Kit, IL-6RB ELISA Kit, 5133400A03Rik ELISA Kit, AA389424 ELISA Kit, BB405851 ELISA Kit, D13Ertd699e ELISA Kit, gp130 ELISA Kit, Ac1055 ELISA Kit, Gp130 ELISA Kit, Il-6rb ELISA Kit, IL-6 ELISA Kit, interleukin 6 signal transducer ELISA Kit, IL6ST ELISA Kit, Il6st ELISA Kit, il6st ELISA Kit

Gen-ID: 3572

UniProt: P40189

Pathways: JAK-STAT Signalweg, Cellular Glucan Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Cancer Immune Checkpoints