POLD1 Antikörper (AA 60-261)

Produktnummer ABIN968287

Produktdetails anti-POLD1 Antikörper

Target: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Bindungsspezifität: AA 60-261

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser POLD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Ratte (Rattus)

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human DNA Polymerase delta aa. 60-261

Klon: 22-D Polymerase delta

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389, ABIN968537

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-POLD1 Antikörper (ABIN968287)

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Wu, Zhang, Zeng, Zhang, Mo, Li, Lee: "Characterization of the p125 subunit of human DNA polymerase delta and its deletion mutants. Interaction with cyclin-dependent kinase-cyclins." in: The Journal of biological chemistry, Vol. 273, Issue 16, pp. 9561-9, (1998) (PubMed).

Sun, Jiang, Zhang, Zhang, Zhou, Li, Toomey, Lee: "Expression and characterization of the small subunit of human DNA polymerase delta." in: The Journal of biological chemistry, Vol. 272, Issue 20, pp. 13013-8, (1997) (PubMed).

Zeng, Jiang, Zhang, Hao, Lee: "DNA polymerase delta is involved in the cellular response to UV damage in human cells." in: The Journal of biological chemistry, Vol. 269, Issue 19, pp. 13748-51, (1994) (PubMed).

Yang, Chang, Zhang, Hao, Zhu, Toomey, Lee: "Molecular cloning of the cDNA for the catalytic subunit of human DNA polymerase delta." in: Nucleic acids research, Vol. 20, Issue 4, pp. 735-45, (1992) (PubMed).

Details zu POLD1

Target: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Andere Bezeichnung: DNA Polymerase delta (POLD1 Produkte)

Synonyme: CDC2 antikoerper, CRCS10 antikoerper, POLD antikoerper, 5949 antikoerper, CAA61369 antikoerper, CG5949 antikoerper, DNA Pol Delta antikoerper, Dm Poldelta antikoerper, Dmel\\CG5949 antikoerper, Poldelta antikoerper, dnapol delta antikoerper, l(3)72Ac antikoerper, cdc2 antikoerper, pold antikoerper, 125kDa antikoerper, Pol delta antikoerper, fla antikoerper, im:7139126 antikoerper, zgc:152688 antikoerper, DNA polymerase delta 1, catalytic subunit antikoerper, DNA-polymerase-delta antikoerper, polymerase (DNA directed), delta 1, catalytic subunit antikoerper, DNA polymerase delta catalytic subunit antikoerper, DNA polymerase delta antikoerper, POLD1 antikoerper, DNApol-delta antikoerper, pold1 antikoerper, LOC100456571 antikoerper, Pold1 antikoerper

Hintergrund: Errors in DNA sequence result from environmental factors or are committed by DNA polymerases during replication. If unchecked, these errors might accumulate genetic damage such that the cell could no longer function. Thus, DNA repair processes involve mechanisms for the excision of damaged sequences and the resynthesis and ligation of the proper sequence. In mammalian cells, this proofreading function rests with DNA polymerase (pol) delta, a heterodimer of a 50kDa subunit, which stimulates pol delta activity in the presence of PCNA (proliferating cell nuclear antigen) and a 125kDa catalytic subunit. The catalytic subunit has 3' to 5' exonuclease activity which distinguishes pol delta from pol alpha and pol beta. Pol delta is also central to DNA replication where it functions in leading strand synthesis at the replication fork. The catalytic subunit is phosphorylated by G1 cyclin-dependent kinase-cyclin complexes and, via its N-terminal 249 amino acids, interacts with cdk2. However, phosphorylation has little or no effect on the activity of pol delta. Thus, DNA polymerase ä is essential for DNA replication and is unique in its ability to replace damaged sequences through the process of DNA excision repair.

Molekulargewicht: 125 kDa

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Chromatin Binding, Synthesis of DNA