POLD1 Antikörper (Catalytic Subunit)

Produktnummer ABIN7150227

Produktdetails anti-POLD1 Antikörper

Target: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Bindungsspezifität: AA 1-123, Catalytic Subunit

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLD1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Kreuzreaktivität: Human

Aufreinigung: >95%, Protein G purified

Immunogen: Recombinant Human DNA polymerase delta catalytic subunit protein (1-123AA)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IP:1:200-1:2000,

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, pH 7.4

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Details zu POLD1

Target: POLD1 (Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa (POLD1))

Andere Bezeichnung: POLD1 (POLD1 Produkte)

Synonyme: CDC2 antikoerper, CRCS10 antikoerper, POLD antikoerper, 5949 antikoerper, CAA61369 antikoerper, CG5949 antikoerper, DNA Pol Delta antikoerper, Dm Poldelta antikoerper, Dmel\\CG5949 antikoerper, Poldelta antikoerper, dnapol delta antikoerper, l(3)72Ac antikoerper, cdc2 antikoerper, pold antikoerper, 125kDa antikoerper, Pol delta antikoerper, fla antikoerper, im:7139126 antikoerper, zgc:152688 antikoerper, DNA polymerase delta 1, catalytic subunit antikoerper, DNA-polymerase-delta antikoerper, polymerase (DNA directed), delta 1, catalytic subunit antikoerper, DNA polymerase delta catalytic subunit antikoerper, DNA polymerase delta antikoerper, POLD1 antikoerper, DNApol-delta antikoerper, pold1 antikoerper, LOC100456571 antikoerper, Pold1 antikoerper

Hintergrund:

Background: As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3\\\'- to 5\\\'-exonuclease activities (PubMed:16510448, PubMed:19074196, PubMed:20334433, PubMed:24035200, PubMed:24022480). Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4 (PubMed:19074196, PubMed:20334433). Although both Pol-delta3 and Pol-delta4 process Okazaki fragments in vitro, Pol-delta3 may be better suited to fulfill this task, exhibiting near-absence of strand displacement activity compared to Pol-delta4 and stalling on encounter with the 5\\\'-blocking oligonucleotides. Pol-delta3 idling process may avoid the formation of a gap, while maintaining a nick that can be readily ligated (PubMed:24035200). Along with DNA polymerase kappa, DNA polymerase delta carries out approximately half of nucleotide excision repair (NER) synthesis following UV irradiation (PubMed:20227374). Under conditions of DNA replication stress, in the presence of POLD3 and POLD4, may catalyze the repair of broken replication forks through break-induced replication (BIR) (PubMed:24310611). Involved in the translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites (PubMed:19074196).

Aliases: Polymerase (DNA directed) delta 1 catalytic subunit antibody, CDC2 antibody, CDC2 homolog antibody, CRCS10 antibody, DNA directed DNA polymerase delta 1 antibody, DNA directed polymerase delta 1 antibody, DNA pol delta 1 antibody, DNA polymerase delta catalytic subunit antibody, DNA polymerase subunit delta p125 antibody, DPOD1_HUMAN antibody, MDPL antibody, POLD antibody, POLD 1 antibody, POLD1 antibody, Polymerase (DNA directed) delta 1 catalytic subunit 125 kDa antibody, Polymerase (DNA) delta 1 catalytic subunit antibody, Polymerase DNA directed delta 1 catalytic subunit 125kD antibody, polymerase, DNA, delta antibody

UniProt: P28340

Pathways: Telomere Maintenance, DNA Reparatur, DNA Replication, Chromatin Binding, Synthesis of DNA