PHLPP1 Antikörper (N-Term)

Produktnummer ABIN6991746

Produktdetails anti-PHLPP1 Antikörper

Target: PHLPP1 (PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1))

Bindungsspezifität: AA 250-300, N-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PHLPP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: At least four isoforms are known to exist, this antibody will only detect the largest isoform. PHLPP1 antibody is predicted to not cross react with PHLPP2.

Aufreinigung: PHLPP1 Antibody is affinity chromatography purified via peptide column.

Immunogen: PHLPP1 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PHLPP1. The immunogen is located within amino acids 250 - 300 of PHLPP1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: PHLPP1 antibody can be used for detection of PHLPP1 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PHLPP1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: PHLPP1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Details zu PHLPP1

Target: PHLPP1 (PH Domain and Leucine Rich Repeat Protein Phosphatase 1 (PHLPP1))

Andere Bezeichnung: PHLPP1 (PHLPP1 Produkte)

Synonyme: PHLPP antikoerper, PLEKHE1 antikoerper, SCOP antikoerper, AI836256 antikoerper, Phlpp antikoerper, Plekhe1 antikoerper, mKIAA0606 antikoerper, Scop antikoerper, PH domain and leucine rich repeat protein phosphatase 1 antikoerper, PHLPP1 antikoerper, phlpp1 antikoerper, Phlpp1 antikoerper

Hintergrund: PHLPP1 Antibody: PHLPP1 is a member of the serine/threonine phosphatase family, which are important regulators of Akt serine-threonine kinases (AKT1, AKT2, AKT3) and conventional/novel protein kinase C (PKC) isoforms. PHLPP1 and PHLPP2 have a similar domain structure and have been shown to dephosphorylate and inactivate, distinct Akt isoforms, at one of the two critical phosphorylation sites required for activation: Serine473. PHLPP2 dephosphorylates AKT1 and AKT3, whereas PHLPP1 is specific for AKT2 and AKT3. PHLPP1 promotes apoptosis and may act as a tumor suppressor. Increased expression of PHLPP1 may also play a role in obesity and type 2 diabetes by interfering with Akt-mediated insulin signaling.

Molekulargewicht: 189 kDa

Gen-ID: 23239

NCBI Accession: NP_919431

UniProt: O60346

Pathways: PI3K-Akt Signalweg, Fc-epsilon Rezeptor Signalübertragung, Neurotrophin Signalübertragung