EPAS1 Antikörper (Internal Region)

Produktnummer ABIN6655142

Produktdetails anti-EPAS1 Antikörper

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EPAS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Aufreinigung: Anti-HIF2α Antibody was purified by Protein G chromatography. This monoclonal antibody is specific for human HIF2α protein. A BLAST analysis was used to suggest cross-reactivity with HIF2α from human based on 100% homology with the immunizing sequence. Cross-reactivity with HIF2α from other sources has not been determined. Oxidative Stress/Cell Signaling research.

Immunogen:

Immunogen: HIF2 alpha Antibody was produced in mice by repeated immunizations raised against a recombinant protein corresponding to an internal region of human EPAS-1 (HIF2alpha).

Immunogen Type: Recombinant Protein

Klon: EP190B

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: User Optimized

Application Note: Anti-HIF2α Antibody is recommended for use in ELISA, WB, IHC and IF microscopy. Antibody is provided in PBS pH 7.4. Specific conditions for reactivity should be optimized by the end user.

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:200

Western Blot Dilution: 1:100 - 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer:

Stabilizer: 50 % (v/v) Glycerol

0.09 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Details zu EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Andere Bezeichnung: HIF2 alpha (EPAS1 Produkte)

Synonyme: EPAS1 antikoerper, ECYT4 antikoerper, HIF2A antikoerper, HLF antikoerper, MOP2 antikoerper, PASD2 antikoerper, bHLHe73 antikoerper, HIF-2alpha antikoerper, HRF antikoerper, Hif2a antikoerper, bhlhe73 antikoerper, ecyt4 antikoerper, hif2 antikoerper, hif2a antikoerper, hif2alpha antikoerper, hlf antikoerper, mop2 antikoerper, pasd2 antikoerper, EPAS antikoerper, EPAS-1 antikoerper, endothelial PAS domain protein 1 antikoerper, endothelial PAS domain protein 1 L homeolog antikoerper, EPAS1 antikoerper, epas1 antikoerper, Epas1 antikoerper, epas1.L antikoerper

Hintergrund:

Synonyms: mouse anti-HIF2 alpha Antibody, mouse anti-HIF2a Antibody, ECYT4, EPAS1, HIF2alpha, HIF1 Alpha like factor, HLF, Hypoxia inducible factor 2 alpha, MOP2, PASD2, Endothelial PAS domain-containing protein 1, Basic-helix-loop-helix-PAS protein MOP2, Class E basic helix-loop-helix protein 73

Background: Members of the hypoxia-inducible factor (HIF) family of transcription factors regulate the cellular response to hypoxia. HIF2-Alpha is involved in catecholamine homeostasis, vascular remodeling, physiological angiogenesis and adipogenesis. It is overexpressed in many cancerous tissues, but its exact role in tumour progression remains to be clarified. Studies suggest that in the case of non-small cell lung cancer, HIF2-Alpha actually is a promoter of tumor growth and progression in a solid tumor. Other data suggests that HIF2-Alpha is an important regulator of innate immunity, and therefore may be useful in the therapeutic target for treating inflammatory disorders and cancer.

Gene Name: EPAS1

Gen-ID: 2034

NCBI Accession: NP_001421

UniProt: Q99814

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effekt