Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Verwendungszweck
Axl is a protein encoded by the AXL gene which is approximately 98,3 kDa. Axl is localised to the cell membrane. It is involved in the GPCR pathway, ERK signalling, actin nucleation by ARP-WASP complex and activation of cAMP-dependent PKA. This protein falls under the Tyro3-Axl-Mer receptor tyrosine kinase subfamily. It transduces signals from the extracellular matrix into the cytoplasm by binding to the vitamin K-dependent protein growth arrest-specific 6. Axl is expressed in primary colon tumors but weakly expressed in normal colon tissue. Mutations in the AXL gene may result in lymphocytic choriomeningitis, femoral neuropathy and Borna disease. STJ90939 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This primary antibody specifically binds to endogenous Axl protein which only binds about Y691 when Y691 is phosphorylated.
Spezifität
Phospho-Axl (Y691) Polyclonal Antibody detects endogenous levels of Axl protein only when phosphorylated at Y691.
Produktmerkmale
Rabbit polyclonal to Phospho-Axl (Y691).
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Immunogen
Synthesized peptide derived from human Axl around the phosphorylation site of Y691.