LATS1 Antikörper (Internal Region)

Produktnummer ABIN6262920

Produktdetails anti-LATS1 Antikörper

Target: LATS1 (LATS, Large Tumor Suppressor, Homolog 1 (Drosophila) (LATS1))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LATS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: LATS1/2 Antibody detects endogenous levels of total LATS1/2.

Homologie: Pig,Bovine,Horse,Sheep,Rabbit

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human LATS1/2, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:1000-3000, IHC 1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu LATS1

Target: LATS1 (LATS, Large Tumor Suppressor, Homolog 1 (Drosophila) (LATS1))

Andere Bezeichnung: LATS1 (LATS1 Produkte)

Synonyme: WARTS antikoerper, wts antikoerper, AW208599 antikoerper, Lats antikoerper, si:dkey-181i3.5 antikoerper, wu:fc19a11 antikoerper, zgc:123219 antikoerper, lats1 antikoerper, RGD1564085 antikoerper, warts antikoerper, large tumor suppressor kinase 1 antikoerper, large tumor suppressor antikoerper, large tumor suppressor kinase 1 S homeolog antikoerper, LATS1 antikoerper, Lats1 antikoerper, lats1 antikoerper, lats1.S antikoerper

Hintergrund:

Description: Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. Plays a role in mammary gland epithelial cells differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1 (PubMed:28068668).

Gene: LATS1

Molekulargewicht: 160 kDa

Gen-ID: 9113

UniProt: O95835, Q9NRM7

Pathways: Regulation of Actin Filament Polymerization, Maintenance of Protein Location