CDK7 Antikörper (Internal Region)

Produktnummer ABIN6260718

Produktdetails anti-CDK7 Antikörper

Target: CDK7 (Cyclin-Dependent Kinase 7 (CDK7))

Bindungsspezifität: Internal Region

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDK7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: CDK7 Antibody detects endogenous levels of total CDK7.

Homologie: Pig,Zebrafish,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus

Aufreinigung: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human CDK7, corresponding to a region within the internal amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000, IHC: 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20 °C. Stable for 12 months from date of receipt.

Haltbarkeit: 12 months

Details zu CDK7

Target: CDK7 (Cyclin-Dependent Kinase 7 (CDK7))

Andere Bezeichnung: CDK7 (CDK7 Produkte)

Synonyme: CAK1 antikoerper, CDKN7 antikoerper, HCAK antikoerper, MO15 antikoerper, STK1 antikoerper, p39MO15 antikoerper, AI323415 antikoerper, AI528512 antikoerper, C230069N13 antikoerper, Cdkn7 antikoerper, Crk4 antikoerper, CD 7 antikoerper, CDK7 antikoerper, CG3319 antikoerper, DmCDK7 antikoerper, DmCdk7 antikoerper, DmMO15 antikoerper, DmMo15 antikoerper, Dmcdk7 antikoerper, Dmel\\CG3319 antikoerper, Mo15 antikoerper, cdk-7 antikoerper, cdk7 antikoerper, dCdk7 antikoerper, p40[MO15] antikoerper, p40MO15 antikoerper, stk1 antikoerper, zgc:85821 antikoerper, cak1 antikoerper, cdkn7 antikoerper, p39mo15 antikoerper, cyclin dependent kinase 7 antikoerper, cyclin-dependent kinase 7 antikoerper, Cyclin-dependent kinase 7 antikoerper, cyclin-dependent kinase 7 L homeolog antikoerper, CDK7 antikoerper, Cdk7 antikoerper, cdk7.L antikoerper, cdk7 antikoerper

Hintergrund:

Description: Serine/threonine kinase involved in cell cycle control and in RNA polymerase II-mediated RNA transcription. Cyclin-dependent kinases (CDKs) are activated by the binding to a cyclin and mediate the progression through the cell cycle. Each different complex controls a specific transition between 2 subsequent phases in the cell cycle. Required for both activation and complex formation of CDK1/cyclin-B during G2-M transition, and for activation of CDK2/cyclins during G1-S transition (but not complex formation). CDK7 is the catalytic subunit of the CDK-activating kinase (CAK) complex. Phosphorylates SPT5/SUPT5H, SF1/NR5A1, POLR2A, p53/TP53, CDK1, CDK2, CDK4, CDK6 and CDK11B/CDK11. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation, thus regulating cell cycle progression. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminal domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Phosphorylation of POLR2A in complex with DNA promotes transcription initiation by triggering dissociation from DNA. Its expression and activity are constant throughout the cell cycle. Upon DNA damage, triggers p53/TP53 activation by phosphorylation, but is inactivated in turn by p53/TP53, this feedback loop may lead to an arrest of the cell cycle and of the transcription, helping in cell recovery, or to apoptosis. Required for DNA-bound peptides-mediated transcription and cellular growth inhibition.

Gene: CDK7

Molekulargewicht: 39kDa

Gen-ID: 1022

UniProt: P50613

Pathways: Zellzyklus, DNA Reparatur, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, M Phase