BAX Antikörper (AA 3-16)

Produktnummer ABIN6252364

Produktdetails anti-BAX Antikörper

Target: BAX (BCL2-Associated X Protein (BAX))

Bindungsspezifität: AA 3-16

Reaktivität: Human, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BAX Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Spezifität: Does not react with mouse and rat

Aufreinigung: Protein G affinity chromatography

Immunogen: Amino acids 3-16 (GSGEQPRGGGPTSS) from the human protein were used as the immunogen for this anti-Bax antibody.

Klon: SPM336

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 1-2 μg/mL

Western blot: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1)

Prediluted format : incubate for 30 min at RT (2)

The optimal dilution of the anti-Bax antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Andere Bezeichnung: Bax (BAX Produkte)

Synonyme: BAX-ALPHA antikoerper, bax-A antikoerper, xBax antikoerper, xlbax antikoerper, BAX antikoerper, bax antikoerper, fj16e01 antikoerper, wu:fc50b10 antikoerper, wu:fj16e01 antikoerper, BCL2L4 antikoerper, zgc:112983 antikoerper, BCL2 associated X, apoptosis regulator antikoerper, BCL2-associated X protein L homeolog antikoerper, BCL2-associated X protein antikoerper, bcl2-associated X protein, a antikoerper, bcl2-associated X protein, b antikoerper, BAX antikoerper, bax.L antikoerper, bax antikoerper, baxa antikoerper, Bax antikoerper, baxb antikoerper

Hintergrund: Recognizes a protein of 21  kDa, identified as the Bax protein. This mAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.

Gen-ID: 581

UniProt: Q07812

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response