Androgen Receptor Antikörper (AA 151-350)

Produktnummer ABIN6251809

Produktdetails anti-Androgen Receptor Antikörper

Target: Androgen Receptor (AR)

Bindungsspezifität: AA 151-350

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Androgen Receptor Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Spezifität: Does not react with Mouse

Aufreinigung: Protein G affinity chromatography

Immunogen: A recombinant partial-length human AR protein corresponding to amino acids 151-350 was used as the immunogen for the Androgen Receptor antibody.

Klon: DHTR-882

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 0.5-1 μg/million cells in 0.1ml

Immunofluorescence: 0.5-1 μg/mL

Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1)

Prediluted format : incubate for 30 min at RT (2)

Optimal dilution of the Androgen Receptor antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Tris with 1  mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.2 mg/mL

Buffer: PBS with 0.1 mg/mL BSA and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and Store at -20C. Avoid freez-thaw cycles.

Details zu Androgen Receptor

Target: Androgen Receptor (AR)

Andere Bezeichnung: Androgen Receptor (AR Produkte)

Synonyme: AIS antikoerper, DHTR antikoerper, HUMARA antikoerper, HYSP1 antikoerper, KD antikoerper, NR3C4 antikoerper, SBMA antikoerper, SMAX1 antikoerper, TFM antikoerper, AW320017 antikoerper, Tfm antikoerper, Andr antikoerper, AR antikoerper, androgen receptor antikoerper, AR antikoerper, Ar antikoerper, ar antikoerper

Hintergrund: Recognizes a protein of 110  kDa, which is identified as androgen receptor (AR). It reacts with full length, and the newly described A form of the receptor. It does not cross react with estrogen, progesterone, or glucocorticoid receptors. The expression of AR is reportedly inversely correlated with histologic grade i.e. well differentiated prostate tumors show higher expression than the poorly differentiated tumors. In prostate cancer, AR has been proposed, as a marker of hormone-responsiveness and thus it may be useful in identifying patients likely to benefit from anti-androgen therapy. Anti-androgen receptor has been useful clinically in differentiating morpheaform basal cell carcinoma (mBCC) from desmoplastic trichoepithelioma (DTE) in the skin. This mAb is superb for staining of formalin/paraffin tissues.

Gen-ID: 367

UniProt: P10275

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding