ZAP70 Antikörper (AA 1-254) (CF®647)

Produktnummer ABIN6189279

Produktdetails anti-ZAP70 Antikörper (CF®647)

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Bindungsspezifität: AA 1-254

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ZAP70 Antikörper ist konjugiert mit CF®647

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Verwendungszweck: Mouse Monoclonal anti-ZAP70 (2F3.2), CF647 Conjugate

Produktmerkmale: ZAP70 is a 70 kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene.In Western blotting of whole cell lysates of normal peripheral blood mononuclear cells, the antibody labels a band corresponding to ZAP70. In Western blotting of whole cell lysates of CD19-positive purified leukemia cells from patients with Ig-unmutated and Ig-mutated CLL, the antibody labels a band corresponding to ZAP70 in the Ig-unmutated CLL samples, whereas no band is observed in the Ig-mutated CLL samples. In Western blotting of cell lysates of Jurkat cells (T-lymphoblastic cell line), the antibody labels a band of 70 kDa protein. In Western blotting of cell lysates of A431 cells (carcinoma cell line), no band is observed. ZAP70 protein is expressed in leukemic cells of approximately 25 % of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant ZAP-70 protein including residues 1-254 and encompassing SH2 domains of human ZAP70

Klon: 2F3-2

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Jurkat cells. Tonsil or lymph node.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu ZAP70

Target: ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))

Andere Bezeichnung: ZAP70 (ZAP70 Produkte)

Synonyme: SRK antikoerper, STCD antikoerper, STD antikoerper, TZK antikoerper, ZAP-70 antikoerper, 70kDa antikoerper, AI327364 antikoerper, Srk antikoerper, mrtle antikoerper, mur antikoerper, zap70 antikoerper, MGC80670 antikoerper, MGC88962 antikoerper, ZAP70 antikoerper, zgc:110383 antikoerper, zeta chain of T-cell receptor associated protein kinase 70 antikoerper, putative tyrosine-protein kinase zap-70 antikoerper, zeta-chain (TCR) associated protein kinase antikoerper, zeta chain of T cell receptor associated protein kinase 70 antikoerper, zeta-chain (TCR) associated protein kinase 70kDa antikoerper, zeta chain of T cell receptor associated protein kinase 70kDa L homeolog antikoerper, zeta chain of T cell receptor associated protein kinase 70kDa antikoerper, tyrosine-protein kinase ZAP-70 antikoerper, ZAP70 antikoerper, Smp_149470 antikoerper, Zap70 antikoerper, zap70.L antikoerper, zap70 antikoerper, LOC707555 antikoerper, LOC100342021 antikoerper, LOC100390922 antikoerper, LOC100542877 antikoerper

Hintergrund: Selective T cell defect, SRK, STD, Syk-related tyrosine kinase, Tyrosine-protein kinase ZAP-70,TZK, Zeta chain associated protein kinase 70  kDa

Molekulargewicht: 70 kDa

Gen-ID: 7535, 234569

UniProt: P43403

Pathways: T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway