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ZAP70 Antikörper (pTyr319)

ZAP70 Reaktivität: Human, Maus, Ratte WB, IHC, ELISA, IF, ICC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN6255884
  • Target Alle ZAP70 Antikörper anzeigen
    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
    Bindungsspezifität
    • 40
    • 40
    • 26
    • 20
    • 20
    • 16
    • 14
    • 10
    • 10
    • 9
    • 8
    • 8
    • 8
    • 8
    • 7
    • 7
    • 6
    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr319
    Reaktivität
    • 296
    • 128
    • 48
    • 8
    • 7
    • 7
    • 6
    • 6
    • 5
    • 3
    • 3
    • 2
    • 1
    Human, Maus, Ratte
    Wirt
    • 219
    • 73
    • 2
    • 2
    • 1
    Kaninchen
    Klonalität
    • 197
    • 99
    Polyklonal
    Konjugat
    • 146
    • 18
    • 16
    • 16
    • 12
    • 8
    • 8
    • 8
    • 7
    • 7
    • 7
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser ZAP70 Antikörper ist unkonjugiert
    Applikation
    • 201
    • 131
    • 101
    • 69
    • 53
    • 53
    • 48
    • 27
    • 27
    • 22
    • 13
    • 12
    • 6
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
    Spezifität
    Phospho-ZAP70 (Tyr319) Antibody detects endogenous levels of ZAP70 only when phosphorylated at Tyrosine 319.
    Homologie
    Pig,Bovine,Horse,Sheep,Rabbit,Dog,Chicken,Xenopus
    Aufreinigung
    The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
    Immunogen
    A synthesized peptide derived from human ZAP70 around the phosphorylation site of Tyr319.
    Isotyp
    IgG
    Top Product
    Discover our top product ZAP70 Primärantikörper
  • Applikationshinweise
    WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store at -20 °C. Stable for 12 months from date of receipt.
    Haltbarkeit
    12 months
  • Target
    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
    Andere Bezeichnung
    ZAP70 (ZAP70 Produkte)
    Synonyme
    SRK antikoerper, STCD antikoerper, STD antikoerper, TZK antikoerper, ZAP-70 antikoerper, 70kDa antikoerper, AI327364 antikoerper, Srk antikoerper, mrtle antikoerper, mur antikoerper, zap70 antikoerper, MGC80670 antikoerper, MGC88962 antikoerper, ZAP70 antikoerper, zgc:110383 antikoerper, zeta chain of T-cell receptor associated protein kinase 70 antikoerper, putative tyrosine-protein kinase zap-70 antikoerper, zeta-chain (TCR) associated protein kinase antikoerper, zeta chain of T cell receptor associated protein kinase 70 antikoerper, zeta-chain (TCR) associated protein kinase 70kDa antikoerper, zeta chain of T cell receptor associated protein kinase 70kDa L homeolog antikoerper, zeta chain of T cell receptor associated protein kinase 70kDa antikoerper, tyrosine-protein kinase ZAP-70 antikoerper, ZAP70 antikoerper, Smp_149470 antikoerper, Zap70 antikoerper, zap70.L antikoerper, zap70 antikoerper, LOC707555 antikoerper, LOC100342021 antikoerper, LOC100390922 antikoerper, LOC100542877 antikoerper
    Hintergrund

    Description: Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).

    Gene: ZAP70

    Molekulargewicht
    70kDa
    Gen-ID
    7535
    UniProt
    P43403
    Pathways
    T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway
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