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ZAP70 Antikörper (zeta-Chain (TCR) Associated Protein Kinase 70kDa) C-Term Primary Antibody

ZAP70 Reaktivität: Human ICC, FACS, WB Wirt: Maus Monoclonal ZAP-03 unconjugated
Pubmed (1 reference)
Produktnummer ABIN125756
$367.36
Zzgl. Versandkosten $45.00
0.1 mg
local_shipping Lieferung nach: Vereinigte Staaten von Amerika
Lieferung in 5 bis 7 Werktagen
  • Target Alle ZAP70 Antikörper anzeigen
    ZAP70
    Bindungsspezifität
    • 39
    • 34
    • 32
    • 21
    • 18
    • 16
    • 15
    • 11
    • 11
    • 10
    • 10
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    • 2
    • 2
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    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
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    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 323
    • 138
    • 52
    • 8
    • 7
    • 6
    • 6
    • 5
    • 5
    • 3
    • 3
    • 2
    • 1
    Human
    Wirt
    • 219
    • 95
    • 8
    • 2
    • 1
    Maus
    Klonalität
    • 219
    • 105
    Monoklonal
    Konjugat
    • 154
    • 19
    • 19
    • 15
    • 13
    • 9
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Dieser ZAP70 Antikörper ist unkonjugiert
    Applikation
    • 179
    • 146
    • 129
    • 84
    • 68
    • 53
    • 53
    • 36
    • 35
    • 35
    • 13
    • 13
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 2
    Immunocytochemistry (ICC), Flow Cytometry (FACS), Western Blotting (WB)
    Spezifität
    The antibody ZAP-03 reacts with ZAP-70, a 70 kDa protein tyrosine kinase expressed in T and NK cells.
    ZAP-70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
    Kreuzreaktivität (Details)
    Human
    Aufreinigung
    Purified by protein-A affinity chromatography
    Reinheit
    > 95 % (by SDS-PAGE)
    Immunogen
    Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP-70 with histidine tag
    Klon
    ZAP-03
    Isotyp
    IgG1
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Do not freeze.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
  • Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, (2011) (PubMed).

  • Target
    ZAP70
    Andere Bezeichnung
    ZAP-70 (ZAP70 Produkte)
    Synonyme
    SRK, STCD, STD, TZK, ZAP-70, 70kDa, AI327364, Srk, mrtle, mur, zap70, MGC80670, MGC88962, ZAP70, zgc:110383, zeta chain of T-cell receptor associated protein kinase 70, putative tyrosine-protein kinase zap-70, zeta-chain (TCR) associated protein kinase, zeta chain of T cell receptor associated protein kinase 70, zeta-chain (TCR) associated protein kinase 70kDa, zeta chain of T cell receptor associated protein kinase 70kDa L homeolog, zeta chain of T cell receptor associated protein kinase 70kDa, tyrosine-protein kinase ZAP-70, ZAP70, Smp_149470, Zap70, zap70.L, zap70, LOC707555, LOC100342021, LOC100390922, LOC100542877
    Hintergrund
    The ZAP-70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP-70 gene mutations resulting in lower ZAP-70 protein expression levels or expression of catalytically inactive ZAP-70 proteins, have been identified. ZAP-70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency.In patients with chronic lymphocytic leukemia (B-CLL), ZAP-70 expression on B cell was shown to be correlated with disease progression and survival. ZAP-70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP-70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP-70 on Tyr493 in the activation loop. ZAP-70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP-70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.
    Pathways
    T-Zell Rezeptor Signalweg, Ubiquitin Proteasome Pathway
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