Transcription factor E2F1 Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Transcription factor E2F1 Antibody was raised against synthetic peptide QEVTSEEENRATDS found at an internal region of Transcription factor E2F1.
E2F1
Reaktivität: Human
WB, ELISA, IF (cc), IF (p), FACS, IHC (p), ICC, IHC (fro)
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 60 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 46.9 kDa according to NP_005216.1). Recommended concentration: 1-3μ,g/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Transcription factor E2F1 is supplied in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot and store at -20°C. Minimize freezing and thawing.