Collagen IV Antikörper
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- Target Alle Collagen IV (COL4) Antikörper anzeigen
- Collagen IV (COL4) (Collagen, Type IV (COL4))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Collagen IV Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Radioimmunoassay (RIA)
- Spezifität
- Human collagen type IV 100 % human collagen types I, II, III, and V <0.1 % human fibronectin and laminin < 0.1 % No cross-reactivity with bovine
- Keine Kreuzreaktivität
- Rind (Kuh), Maus, Schwein, Ratte
- Kreuzreaktivität (Details)
- Human, no cross-reactivity with bovine, mouse, rat and porcine collagens type IV
- Produktmerkmale
- Type IV Collagen is a non-fibrillary network of different alpha-chains: alpha 1(IV) to-alpha 6(IV). It is typically found in basal membranes of different organs (e.g. skin, lens, lung, kidney). Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type IV 100 % , human collagen types I, II, III, and V <0.1 %, human fibronectin and laminin < 0.1 % (RIA at 1:10000 dilution).
- Aufreinigung
- Protein G affinity purified antibody prod. in nude mice.
- Immunogen
- Purified collagen type IV from human placenta
- Klon
- 24D1
- Isotyp
- IgG1
- Top Product
- Discover our top product COL4 Primärantikörper
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- Applikationshinweise
- After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Human skin, placenta or lens capsule,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) IFA ? 1:320, IHC(P) ? 1:4000, ELISA ? 1:4000 (OD ? 500)
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Konzentration
- Lot specific
- Buffer
- phosphate buffered solution, no BSA and preservative added!
- Konservierungsmittel
- Without preservative
- Lagerung
- -20 °C
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- Target
- Collagen IV (COL4) (Collagen, Type IV (COL4))
- Andere Bezeichnung
- Collagen Type IV (COL4 Produkte)
- Synonyme
- collagen, type IV antikoerper, Let-2 antikoerper
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