Collagen IV Antikörper

Produktnummer ABIN961130

Produktdetails anti-Collagen IV Antikörper

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Collagen IV Antikörper ist unkonjugiert

Applikation: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Marke: IHC-plus™

Spezifität: Human Type IV collagen. Cross-reactivity: Human collagen type I: 1 % Human collagen type II: <0.5 %, Human collagen type III: <0.5 %, Human collagen type IV: 100 %, Human collagen type V: 7 %, Human fibronectin: <0.5 %, Human albumin: <0.5 %, Human immunoglobulin <0.5 %.

Aufreinigung: Affinity purified

Immunogen: Human placental collagen type IV.

Type of Immunogen: Purified protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1:3000), IF, IHC, IHC-Fr, IHC-P (1:100)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3654 was determined to be 1:100. Suitable for use in ELISA, Indirect immunofluorescence, indirect immunohistochemistry and Dot Blotting (not suitable for Western Blot). ELISA: 1:3000. Indirect immunofluorescent visualization of collagen type IV on cryostat sections of human tissue: 1:20-1:40. Indirect Immunohistochemistry: 1:20-1:40. fresh frozen or acetone fixed specimens. Dot Blotting: 1:300. not recommended for Western Blots.

Not recommended for: WB

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, mannitol, dextran, no preservatives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Andere Bezeichnung: Collagen IV (COL4 Produkte)

Synonyme: collagen, type IV antikoerper, Let-2 antikoerper