Collagen IV Antikörper

Produktnummer ABIN7212370

Der Maus Monoklonal anti-Collagen IV Antikörper (Klon 8E5) (ABIN7212370) detektiert spezifisch Collagen IV in IHC und IF. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für Collagen IV Antikörper (ABIN7212370)

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Collagen IV Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: 8E5

Produktdetails anti-Collagen IV Antikörper

Verwendungszweck: Mouse Anti-Collagen IV Mouse Monoclonal Antibody (8E5)

Spezifität: The antibody detects endogenous Collagen IV protein.

Aufreinigung: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthetic Peptide of Collagen IV

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IF 1:50-200,IHC 1:50-300

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu Collagen IV

Target: Collagen IV (COL4) (Collagen, Type IV (COL4))

Andere Bezeichnung: Collagen IV

Hintergrund: Collagen alpha-1(IV) chain [Cleaved into: Arresten]COL4A1 (collagen type IV alpha 1 chain) encodes a type IV collagen alpha protein. Type IV collagen proteins are integral components of basement membranes. COL4A1 shares a bidirectional promoter with a paralogous gene on the opposite strand. The protein consists of an amino-terminal 7S domain, a triple-helix forming collagenous domain, and a carboxy-terminal non-collagenous domain. It functions as part of a heterotrimer and interacts with other extracellular matrix components such as perlecans, proteoglycans, and laminins. In addition, proteolytic cleavage of the non-collagenous carboxy-terminal domain results in a biologically active fragment known as arresten, which has anti-angiogenic and tumor suppressor properties. Mutations in COL4A1 cause porencephaly, cerebrovascular disease, and renal and muscular defects. Alternative splicing results in multiple transcript variants.

Molekulargewicht: 161kD

Gen-ID: 1282