Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC)
Verwendungszweck
Mouse monoclonal antibody raised against partial recombinant ZAP70.
Spezifität
This antibody reacts with ZAP70, a 70 KDa protein tyrosine kinase expressed in T and NK cells. ZAP70 is a molecule susceptible to degradation. It is recommended to use freshly prepared cell lysates (protease inhibitors are essential) to avoid non-specific staining of degradation products.
Kreuzreaktivität
Human
Immunogen
Recombinant His fusion protein corresponding to 160 amino acids of human ZAP70.
Flow Cytometry (2-5 μg/mL) Western Blot (0.5 μg/mL) The optimal working dilution should be determined by the end user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
In PBS, pH 7.4 (0.09 % sodium azide)
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C
Informationen zur Lagerung
Store at 4°C. Do not freeze. Aliquot to avoid repeated freezing and thawing.
Van Lierop, Wijnands, Loke, Emmer, Lukassen, Braat, van der Meer, Mosselman, Joosten: "Detection of HLA-G by a specific sandwich ELISA using monoclonal antibodies G233 and 56B." in: Molecular human reproduction, Vol. 8, Issue 8, pp. 776-84, (2002) (PubMed).
Blaschitz, Hutter, Dohr: "HLA Class I protein expression in the human placenta." in: Early pregnancy (Online), Vol. 5, Issue 1, pp. 67-9, (2001) (PubMed).
Target
ZAP70
(zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))