Topoisomerase II alpha Antikörper (AA 1352-1493)

Produktnummer ABIN4949921

Produktdetails anti-Topoisomerase II alpha Antikörper

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Bindungsspezifität: AA 1352-1493

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase II alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Aufreinigung: Protein G affinity chromatography

Immunogen: Amino acids 1352-1493 from the human protein were used as the immunogen for this Topoisomerase II alpha antibody.

Klon: TOP2A-1361

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Topoisomerase II alpha antibody to be titered up or down for optimal performance.\. FACS: 0.5-1 μg/10^6 cells in 0.1ml,IF: 1-2 μg/mL,Western blot: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Topoisomerase II alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Andere Bezeichnung: TOP2A / Topoisomerase II alpha (TOP2A Produkte)

Synonyme: TOP2 antikoerper, TP2A antikoerper, TOP2A antikoerper, Top-2 antikoerper, TOP-2 antikoerper, F12B17.110 antikoerper, F12B17_110 antikoerper, thimet metalloendopeptidase 2 antikoerper, fc08c07 antikoerper, wu:fc08c07 antikoerper, DNA topoisomerase II alpha antikoerper, DNA topoisomerase II, alpha isozyme antikoerper, topoisomerase (DNA) II alpha antikoerper, Zincin-like metalloproteases family protein antikoerper, TOP2A antikoerper, Bm1_08705 antikoerper, Top2a antikoerper, AT5G10540 antikoerper, top2a antikoerper

Hintergrund: This mAb recognizes an ~170  kDa protein which is identified as Topoisomerase IIa, and shows no cross-reaction with Topoisomerase IIb or I. Topo IIa plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo IIa is also implicated in drug resistance of tumor cells.

Gen-ID: 7153

Pathways: Zellzyklus, Mitotic G1-G1/S Phases