DNMT3L Antikörper (N-Term)

Produktnummer ABIN492471

Produktdetails anti-DNMT3L Antikörper

Target: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Bindungsspezifität: AA 1-387, N-Term

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser DNMT3L Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: Detects ~45 kDa protein. No cross-reactivity against DNMT3a or DNMT3B.

Produktmerkmale: Synonyms: DNA (cytosine-5)-methyltransferase 3-like

Aufreinigung: Protein G Chromatography.

Immunogen: Fusion protein amino acids 1-387 of Human DNMT3L (accession number BAA95556).

Klon: S117-9

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western blot: 1 μg/mL1 μg/mL was sufficient for detection of DNMT3L in 10 μg of COS cell transiently transfectedwith Flag-tagged DNMT3L lysate by colorimetric immunoblot analysis using Goatanti-Mouse IgG: HRP as the secondary antibody. Immunoprecipitation: 1.0-10 μg/mLImmunofluorescence: 1.0-10 μg/mLImmunocytochemistry: 0.1-1.0 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 50 % Glycerol as stabilizer and 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu DNMT3L

Target: DNMT3L (TRDMT1) (DNA (Cytosine-5-)-Methyltransferase 3 Like (TRDMT1))

Andere Bezeichnung: DNMT3L (TRDMT1 Produkte)

Synonyme: DNMT3L antikoerper, D6Ertd14e antikoerper, ecat7 antikoerper, DNA methyltransferase 3 like antikoerper, DNA (cytosine-5-)-methyltransferase 3-like antikoerper, DNMT3L antikoerper, Dnmt3l antikoerper

Hintergrund: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue (1-6). Studies show that DNMT3L regulates the activity of DNMT3A and DNMT3B and stimulates their catalytic activities (7). DNMT3L has specifically been linked to the process of carcinogenesis, thru its role in nuclear programming (8).Synonyms: DNA (cytosine-5)-methyltransferase 3-like

Gen-ID: 29947

UniProt: Q9UJW3